Virus isolation from the peripheral blood mononuclear cells of goats
experimentally infected with ovine progressive pneumonia virus

XUE Fei; XIANG Wen-Hua; CHU Gui-Fang; GU Shou-Lin; CHEN Rong-Xian

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February 1997

XUE Fei, XIANG Wen-Hua, CHU Gui-Fang, GU Shou-Lin and CHEN Rong-Xian. Virus isolation from the peripheral blood mononuclear cells of goatsexperimentally infected with ovine progressive pneumonia virus[J]. Virologica Sinica, 1997, 12(1).

Citation: XUE Fei, XIANG Wen-Hua, CHU Gui-Fang, GU Shou-Lin, CHEN Rong-Xian. Virus isolation from the peripheral blood mononuclear cells of goats experimentally infected with ovine progressive pneumonia virus .VIROLOGICA SINICA, 1997, 12(1) : 71.

从实验感染OPPV的山羊外周血单核细胞中分离病毒的研究

1.
中国农业科学院哈尔滨兽医研究所

摘要

用绵羊胎肺细胞与接种绵羊进行性肺炎病毒（OPPV）的山羊外周血单核细胞共同培养的方法可以分离到病毒，这说明OPPV可以感染山羊。用细胞病变观察、间接荧光抗体试验、电镜切片观察和聚合酶链式反应对分离毒进行了鉴定，进一步证实了分离毒为OPPV。分离结果表明这是一种较为敏感的分离方法。绵羊胎肺细胞可传到40多代，且每一代次的细胞都可用于病毒的分离，因此这是一种非常实用的分离OPPV的方法。
- 绵羊进行性肺炎病毒山羊外周血单核细胞绵羊胎肺细胞

Virus isolation from the peripheral blood mononuclear cells of goats experimentally infected with ovine progressive pneumonia virus

1.
Harbin Veterinary Research Institute of Chinese Academy of Agicultural Sciences,Harbin 150001

Abstract

The viruses were isolated by cocultivation of sheep foetal lung cells and the peripheral blood mononuclear cells of goats inoculated with ovine progressive pneumonia virus (OPPV). The result indicates that OPPV can infect goats. The isolated viruses were characterized by observations of cytopathogenic effects, indirect fluorescent antibody assay, electron microscopy, and polymerase chain reaction (PCR), and were further identified as OPPV. The isolation results show that this is a sensitive method of isolating OPPV. Sheep foetal lung cells can be passaged for forty times, and each subculture can be used to isolate virus, so it is a very practical method of isolating OPPV.
- Ovine progressive pneumonia virus Goat Peripheral blood mononuclear cells Sheepfoetal lung cells

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Virus isolation from the peripheral blood mononuclear cells of goats experimentally infected with ovine progressive pneumonia virus

1. Harbin Veterinary Research Institute of Chinese Academy of Agicultural Sciences,Harbin 150001

Abstract: The viruses were isolated by cocultivation of sheep foetal lung cells and the peripheral blood mononuclear cells of goats inoculated with ovine progressive pneumonia virus (OPPV). The result indicates that OPPV can infect goats. The isolated viruses were characterized by observations of cytopathogenic effects, indirect fluorescent antibody assay, electron microscopy, and polymerase chain reaction (PCR), and were further identified as OPPV. The isolation results show that this is a sensitive method of isolating OPPV. Sheep foetal lung cells can be passaged for forty times, and each subculture can be used to isolate virus, so it is a very practical method of isolating OPPV.