以昆虫病毒Flockhousevirus（FHV）外壳蛋白为载体的外源抗原表位表达系统（FHV-RNA2载体系统）．在重组杆状病毒和重组pET系统中构建和表达了SA11Vp4胰酶切割位点两侧和重叠切割位点3个抗原表位氨基酸序列（抗原表位A，aa223~242；抗原表位B，aa243～262；抗原表位C，aa234～251），并对其免疫原性进行了研究。结果表明：这3个抗原表位能诱导动物产生抗同源氨基酸序列的抗体和抗同源病毒（SA11）感染性的血清中和抗体。研究结果提示：RVVp4胰酶切割位点区氨基酸序列除了具有胰酶切割增强病毒感染力外，还具有诱导动物机体产生血清中和抗体的能力，是RV重组抗原表位亚单位疫苗研究中重要的抗原表位氨基酸序列。

In the present study, with a new foreign epitope - presenting system, FHV - RNA2 carrier system, on the precursor capsid protein of an insect virus, flock house virus (FHV) as a carrier, in recombinant baculovirus system and in pET system three epitopes derived from the amino acid residues surrounding the trypsin cleavage site(s) of SAll Vp4 (epitope A, aa 223 to 242;epitope'B, aa 243 to 262; and epitope C, aa 234 to 251 ) was constructed and expressed. The ability of the three epitopes inducing neutralizing antibodies in animals was studies. The results showed that these three epitopes induced production of seral antibodies and neutralizing anbodies in guinea pigs. These findings suggested that the amino acid sequences surrounding the trypsin cleavage site (s) on SAll Vp4 have great significance in the development of epitope - based recombinant ro-tavirus subunit vaccines.Keywoeds: Rotavirus Vp4, Trypsin cleavage site, Epitopes, Neutralizing antibodies, FHV RNA2 carrier system