从非洲绿猴肾细胞株分离高度重复顺序AGMr（HindⅢ）－1基因，以pUC18质粒为载体构建重组质粒pUC18／AGMr（HindⅢ）－1，转化E．coliJM83。酶切分析、SouthernBlot分析和序列分析均证明克隆成功，但所用的第165代Vero细胞株与另一非洲绿猴肾BSC－1细胞株的序列相比较，172个碱基中有6个位点突变。用该重组质粒作探针，对Vero细胞培养的狂犬疫苗作SouthernBlot分析表明，未经纯化的半成品中残余细胞DNA长度约400～5000bp。斑点杂交分析表明，杂交特异性良好，残余细胞DNA最小检出量约4pg。提示AGMr（HindⅢ）－1高度重复顺序可特异地应用于Vero细胞残余DNA检测。

Highly reiterated Sequence AGMr (Hind Ⅲ)-l was isolated from genome of Vero cell line derived from African green monkey and cloned into plasmid pUC18. Sequence analysis confirmed that the insert Sequence is right but 6 out of 172 bp had mutated in the 165th generation of Vero cell. Southern Blot and Dot Blot analysis of rabies vaccine prepared by Vero cell showed that residual cell DNA(RC DNA) displayed heterogeneous size from 400 ～ 5000 bp, and that the amount of RCDNA can be determined specifically and sensitively (minimun 4 pg). The fact that AGMr (Hind Ⅲ )-l Sequence represents about 20% of the genome DNA and repeats 6.8 × 106times and is rare in Primates except in members of the same genus also suggested that the recombinant plasmid could be applied as probe for detecting RC DNA existed in vaccine prepared by Vero cell and maybe used for identifying test of Vero cell.