Molecular cloning and sequencing of ribozyme region of HDV from a serious hepatitis patient

LI Ru-Lin; WANG Hai-Tao; WAN Ze-Sheng; XU De-Zhong

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June 1997

LI Ru-Lin, WANG Hai-Tao, WAN Ze-Sheng and XU De-Zhong. Molecular cloning and sequencing of ribozyme region of HDV from a serious hepatitis patient[J]. Virologica Sinica, 1997, 12(3).

Citation: LI Ru-Lin, WANG Hai-Tao, WAN Ze-Sheng, XU De-Zhong. Molecular cloning and sequencing of ribozyme region of HDV from a serious hepatitis patient .VIROLOGICA SINICA, 1997, 12(3) : 235.

从重症肝炎病人血清分离的丁型肝炎病毒核酶区的克隆与序列分析

1.
第四军医大学流行病教研室军事医学科学院微生物流行病研究所第三军医大学微生物教研室

摘要

从我国一例四川丁型肝炎病毒HDVRNA，丁型肝炎抗原均阳性的重症肝炎患者的血清中，用异硫氰酸胍法提取RNA经过逆转录PCR扩增后获得一长289bp的HDVcDNA片段，将PCR产物回收后直接克隆到PCRTMⅡ质粒，挑出阳性克隆并亚克隆入M13mp19的EcoRⅠ区位点，提取单链进行序列分析，结果显示与已知的中国河南、美国、日本、秘鲁和中国台湾5株HDVcDNA相同片段比较，同源性分别为对97.2％、93％、94％、79％、96％。
- 丁型肝炎病毒逆转录聚合酶链反应核酶核苷酸序列分析

Molecular cloning and sequencing of ribozyme region of HDV from a serious hepatitis patient

Abstract

The cDNA (651nt - 941nt) fragment of an isolate of hepatitis delta virus with 289 bp was determined by cloning and Sequencing, from a HBsAg carrier positive both for anti - HDAg and HDV RNA from China, Sichuan Province, using reverse transcription polymerase chain reaction.It contains the region of HDV ribozyme and its self - cleavage site. Comparing this Sichuan isolate with other known HDV isolates from Henan, US - 1, Japan, Peru and Taiwan, the result showed that the homology of nucbotides is 97. 2 %, 93 %, 94 %, 79 %, 96 %, respectively.
- Hepatitis Delta Virus RT - PCR Ribozyme Sequencing

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Molecular cloning and sequencing of ribozyme region of HDV from a serious hepatitis patient

Abstract: The cDNA (651nt - 941nt) fragment of an isolate of hepatitis delta virus with 289 bp was determined by cloning and Sequencing, from a HBsAg carrier positive both for anti - HDAg and HDV RNA from China, Sichuan Province, using reverse transcription polymerase chain reaction.It contains the region of HDV ribozyme and its self - cleavage site. Comparing this Sichuan isolate with other known HDV isolates from Henan, US - 1, Japan, Peru and Taiwan, the result showed that the homology of nucbotides is 97. 2 %, 93 %, 94 %, 79 %, 96 %, respectively.