Cloning NS3 gene of Hepatitis G Virus from Chinese Patients
of Post·Transfusion Hepatitis and Its Sequence Analysis

XU Dong-Gang; DAI Hao-Yang; NIU Jian-Zhang; HAN Feng-Lian; GAO Xin

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April 1998

XU Dong-Gang, DAI Hao-Yang, NIU Jian-Zhang, HAN Feng-Lian and GAO Xin. Cloning NS3 gene of Hepatitis G Virus from Chinese Patientsof Post·Transfusion Hepatitis and Its Sequence Analysis[J]. Virologica Sinica, 1998, 13(2).

Citation: XU Dong-Gang, DAI Hao-Yang, NIU Jian-Zhang, HAN Feng-Lian, GAO Xin. Cloning NS3 gene of Hepatitis G Virus from Chinese Patients of Post·Transfusion Hepatitis and Its Sequence Analysis .VIROLOGICA SINICA, 1998, 13(2) : 166.

庚型肝炎病毒——中国河北分离株部分NS3区基因序列分析

1.
军事医学科学院基础医学研究所河北省卫生防疫站中国人民解放军３０２医院

摘要

在急慢性输血后肝炎、散发性肝炎及爆发型肝炎中，大约１０～２０％的病人不属于已发现的Ａ～Ｅ型肝炎，提示存在新型肝炎病毒。在美国１９９５年第４６届医学年会上，已有了一些有关庚型肝炎病毒（ＨＧＶ）分子生物学及庚型肝炎血清学方面的摘要报道。１９９６年初Ｌｉｎ...
- 庚型肝炎病毒 NS3基因序列分析

Cloning NS3 gene of Hepatitis G Virus from Chinese Patients of Post·Transfusion Hepatitis and Its Sequence Analysis

1.
1 Institute of Basic Medical Siences．Academy of Military Medical Sicences，Beijing 1008502 Health and Anti—epidemic Station for Hehei Province．Baoding 071000302 Hospital of PLA，Beijing 100039

Abstract

Epitope of HGV NS3 protein was analyzed by Goldkey Program,and according to predicted results,primers of NS3 gene were synthesized.HGV RNA was extracted from patients of post transfusion hepatitis,cDNA fragment of NS3 was amplified by RT PCR.Target gene was cloned into pGEM T vector,and sequence analysis showed that the fragment of NS3 is 591bp,G+C is 58.37%.The homology of nucleotide and reduced amino acid between the isolate and other strains from America and China is 84.26%-86.13%,94.42%-94.92%,respectively.The homology between Chinese strains is higher than that between American.
- Hepatitis G virus NS3 gene Sequence analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning NS3 gene of Hepatitis G Virus from Chinese Patients of Post·Transfusion Hepatitis and Its Sequence Analysis

1. 1 Institute of Basic Medical Siences．Academy of Military Medical Sicences，Beijing 1008502 Health and Anti—epidemic Station for Hehei Province．Baoding 071000302 Hospital of PLA，Beijing 100039

Abstract: Epitope of HGV NS3 protein was analyzed by Goldkey Program,and according to predicted results,primers of NS3 gene were synthesized.HGV RNA was extracted from patients of post transfusion hepatitis,cDNA fragment of NS3 was amplified by RT PCR.Target gene was cloned into pGEM T vector,and sequence analysis showed that the fragment of NS3 is 591bp,G+C is 58.37%.The homology of nucleotide and reduced amino acid between the isolate and other strains from America and China is 84.26%-86.13%,94.42%-94.92%,respectively.The homology between Chinese strains is higher than that between American.