Partial cloning of the genome of non-occluded baculovirus from Penaeus chinensis and preparing the probe for detection

SHI Zheng-Li; HUANG Can-Hua; CHEN Dai-Hua

PDF
Cite
Share

facebook

twitter

google

linkedin

June 1998

SHI Zheng-Li, HUANG Can-Hua and CHEN Dai-Hua. Partial cloning of the genome of non-occluded baculovirus from Penaeus chinensis and preparing the probe for detection[J]. Virologica Sinica, 1998, 13(3).

Citation: SHI Zheng-Li, HUANG Can-Hua, CHEN Dai-Hua. Partial cloning of the genome of non-occluded baculovirus from Penaeus chinensis and preparing the probe for detection .VIROLOGICA SINICA, 1998, 13(3) : 267.

中国对虾杆状病毒基因克隆及探针制备与检测

1.
中国科学院武汉病毒研究所

摘要

从收集的中国对虾（Ｐｅｎａｅｕｓｃｈｉｎｅｎｓｉｓ）病虾样品中分离到一种杆状病毒，经负染在电镜下观察，完整病毒粒子大小为１１０２８０～３２０ｎｍ。病毒核酸经ＥｃｏＲＩ酶切，克隆到ｐＵＣ１８质粒上，经筛选得到两个克隆Ｌ４６、Ｍ１３，克隆片段分别为２．１４ｋｂ和３．５８ｋｂ。将这两个基因片段和对虾白斑综合征杆状病毒（ＷｈｉｔｅＳｐｏｔＳｙｎｄｒｏｍｅＢａｃｕｌｏｖｉｒｕｓ，简称ＷＳＳＶ）基因克隆片段Ａ２６制备探针，共同用点杂交法对我国沿海地区的病虾样品进行检测，以了解我国沿海地区对虾杆状病毒的分布，并确定中国对虾杆状病毒与ＷＳＳＶ的同源性。
- 中国对虾杆状病毒基因克隆核酸探针

Partial cloning of the genome of non-occluded baculovirus from Penaeus chinensis and preparing the probe for detection

1.
Wuhan Institute of Virology,CAS

Abstract

Viral particles similar to non occluded baculoviruses were isolated from penaeid shrimp Penaeus chinensis in China. The size of enveloped virion is 250-300110nm.After extraction,the viral DNA was digested by EcoRⅠ and cloned into the pUC 18. Two clones, L46 and M13,containing inserts of 2.14kb and 3.58kb in size respectively, were obtained.The inserts were labeled by Digoxingenin. Diseased shrimp samples issued from different regions of China were investigated by dot blot hybridization with the previously preparing probes L46, M13 and with A26 probe. All the probes reacted with the tested samples showing baculovirus infection. It indicated that baculovirus from P.chinensis is very close to the WSSV.
- Penaeus chinensis Baculovirus Gene cloning DNA probe

References
Proportional views

PDF

Article Metrics

Article views(3752) PDF downloads(777) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Partial cloning of the genome of non-occluded baculovirus from Penaeus chinensis and preparing the probe for detection

1. Wuhan Institute of Virology,CAS

Abstract: Viral particles similar to non occluded baculoviruses were isolated from penaeid shrimp Penaeus chinensis in China. The size of enveloped virion is 250-300110nm.After extraction,the viral DNA was digested by EcoRⅠ and cloned into the pUC 18. Two clones, L46 and M13,containing inserts of 2.14kb and 3.58kb in size respectively, were obtained.The inserts were labeled by Digoxingenin. Diseased shrimp samples issued from different regions of China were investigated by dot blot hybridization with the previously preparing probes L46, M13 and with A26 probe. All the probes reacted with the tested samples showing baculovirus infection. It indicated that baculovirus from P.chinensis is very close to the WSSV.