Sequence determination of the specific DNA fragment from the genome of strong virulent GA strain of the Marek's disease virus

LU Chun; ZHU Hong-Fei; ZHANG Xun-Hai; CAI Bao-Xiang

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June 1998

LU Chun, ZHU Hong-Fei, ZHANG Xun-Hai and CAI Bao-Xiang. Sequence determination of the specific DNA fragment from the genome of strong virulent GA strain of the Marek's disease virus[J]. Virologica Sinica, 1998, 13(3).

Citation: LU Chun, ZHU Hong-Fei, ZHANG Xun-Hai, CAI Bao-Xiang. Sequence determination of the specific DNA fragment from the genome of strong virulent GA strain of the Marek's disease virus .VIROLOGICA SINICA, 1998, 13(3) : 270.

马立克氏病病毒强毒GA株基因组BamH Ⅰ-L片段的序列分析

1.
南京医科大学微生物教研室南京农业大学动物医学院

摘要

马立克氏病病毒（Ｍａｒｅｋｓｄｉｓｅａｓｅｖｉｒｕｓ，ＭＤＶ）是一种能诱导鸡淋巴组织增生或淋巴肿瘤的细胞结合性疱疹病毒，由此而引起的马立克氏病（Ｍａｒｅｋｓｄｉｓｅａｓｅ，ＭＤ）也是迄今为止唯一可以利用疫苗进行有效控制的肿瘤性疾病。鉴于此，作为研...
- 马立克氏病病毒致瘤毒株核酸序列分析

Sequence determination of the specific DNA fragment from the genome of strong virulent GA strain of the Marek's disease virus

1.
Departmenf of Microbiology，Nanjing Medical University，Nanjing 210029

Abstract

Differentiation of strong virulent and vaccinal Marek's disease virus (MDV) was carried out by using DNA molecular hybridization. DNA probe was made of BamHI L fragment from MDV gene library (strain GA) by labelled with digoxigenin using random priming. And the complete nucleotide sequence of BamHI L fragment was further determined from subcloned DNAs by using double stranded sequencing method. The results of computer analysis showed that, (1) the entire DNA fragment is 2893 bp long and has a base composition of 55.91% G+C;(2) it has no any open reading frames (ORFs); (3) this DNA fragment is possibly involved in transcription of meq gene of MDV.
- Marek\'s disease virus Strong virulent strain Sequencing

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Sequence determination of the specific DNA fragment from the genome of strong virulent GA strain of the Marek's disease virus

1. Departmenf of Microbiology，Nanjing Medical University，Nanjing 210029

Abstract: Differentiation of strong virulent and vaccinal Marek's disease virus (MDV) was carried out by using DNA molecular hybridization. DNA probe was made of BamHI L fragment from MDV gene library (strain GA) by labelled with digoxigenin using random priming. And the complete nucleotide sequence of BamHI L fragment was further determined from subcloned DNAs by using double stranded sequencing method. The results of computer analysis showed that, (1) the entire DNA fragment is 2893 bp long and has a base composition of 55.91% G+C;(2) it has no any open reading frames (ORFs); (3) this DNA fragment is possibly involved in transcription of meq gene of MDV.