HOU Jian-Wen, ZHAO Ye-Feng, TAO Guo-Qiang, JIANG Wei-Min and YANG Wen-Qin. Bioassay of the multiple Autograph californica nucleopolyhedrovirus (AcMNPV) pesticides[J]. Virologica Sinica, 1998, 13(4).
Citation: HOU Jian-Wen, ZHAO Ye-Feng, TAO Guo-Qiang, JIANG Wei-Min, YANG Wen-Qin. Bioassay of the multiple Autograph californica nucleopolyhedrovirus (AcMNPV) pesticides .VIROLOGICA SINICA, 1998, 13(4) : 350.

复配型苜蓿银纹夜蛾核型多角体病毒制剂对两种夜蛾的毒力测定

  • 对防治蔬菜甜菜夜蛾(SpodopteraexiguaHübner)和斜纹夜蛾(ProdenialituraFabricius)为主并兼治菜螟、菜青虫、小菜蛾等鳞翅目害虫的单一型和复配型苜蓿银纹夜蛾核型多角体病毒(AutographacalifornicanuclearpolyhedrosisVirus,AcMNPV)生物杀虫剂进行筛选。毒力测定结果表明:选用单剂的AcMNPVA株(从甜菜夜蛾虫体增殖获得)的毒力(LD50=131.8PIB/头)比AcMNPVB株(从草地贪夜蛾细胞株Sf9中获得)的毒力(LD50=1949.4PIB/头)高14.79倍。AcMNPVA株加苏云金杆菌(Bt)加斜纹夜蛾核型多角体病毒(PlNPV)形成的复配型AcMNPVA株制剂对甜菜夜蛾二龄幼虫的LD50值(21.82~21.4PIB/头)与单剂AcMNPVA株的LD50值(168.84~204.36PIB/头)比值为9.5~7.7倍。同样复配型AcMNPVA株制剂对斜纹夜蛾二龄幼虫LD50(3.16~15.46PIB/头)与单剂PlNPVLD50值(16.02~53.53PIB/头)比值为5.1~3.5倍。说明以Bt和非耙标害虫病毒作为增效剂的复配型AcMNPVA株制剂对两种夜蛾害虫有明显增效作用。以复配型AcMNPVA株制剂形成的蔬菜复合病毒杀虫剂(SNB3)防治蔬菜重要鳞翅目五种抗性害虫取得良好效果。

Bioassay of the multiple Autograph californica nucleopolyhedrovirus (AcMNPV) pesticides

  • The multiple with other biological agent Autographa californica nucleopolyhedrovirus (AcMNPV) pesticide, which is widely used to control Spodoptera exigua and Prodenia litura as well as Artogeia rapat, Hellula untalis, Plutella xylostella , was selected. Tested with 2nd instar S. exigua larvae, the virulence of AcMNPV A strain amplified in S.exigua larvae is higher than that of AcMNPV B strain in Sf9 cells. LD 50 of AcMNPV B (1949.4 PIB/Larva) is 14.79 times more than AcMNPV A (131.8PIB/Larva).The ratio of LD 50 between AcMNPV A (LD 50 =168.84-240.36 PIB/Larva) and AcMNPV A+Bt (LD 50 =1.4329-128.91 PIB/Larva) is 118-1.5 while the ratio between AcMNPV A and AcMNPV A+Bt+PlNPV (LD 50 = 21.82 -21.4 PIB/Larva) is 7.7-9.5. Tested with 2nd instar P.litura larvae, the ratio of LD 50 between PlNPV (LD 50 =16.02-53.53 PIB/Larva)and PlNP V+Bt(LD 50 =2.9-7.76 PIB/Larva ) is 5.5-6.9 while the ratio between PlNPV and PlNPV+Bt+AcMNPV A (LD 50 =3.16-15.469

  • 加载中
  • 加载中

Article Metrics

Article views(3585) PDF downloads(903) Cited by(0)

Related
Proportional views
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Bioassay of the multiple Autograph californica nucleopolyhedrovirus (AcMNPV) pesticides

    • 1. NanjingAgrictdtural College,Nanjing 210038

    Abstract: The multiple with other biological agent Autographa californica nucleopolyhedrovirus (AcMNPV) pesticide, which is widely used to control Spodoptera exigua and Prodenia litura as well as Artogeia rapat, Hellula untalis, Plutella xylostella , was selected. Tested with 2nd instar S. exigua larvae, the virulence of AcMNPV A strain amplified in S.exigua larvae is higher than that of AcMNPV B strain in Sf9 cells. LD 50 of AcMNPV B (1949.4 PIB/Larva) is 14.79 times more than AcMNPV A (131.8PIB/Larva).The ratio of LD 50 between AcMNPV A (LD 50 =168.84-240.36 PIB/Larva) and AcMNPV A+Bt (LD 50 =1.4329-128.91 PIB/Larva) is 118-1.5 while the ratio between AcMNPV A and AcMNPV A+Bt+PlNPV (LD 50 = 21.82 -21.4 PIB/Larva) is 7.7-9.5. Tested with 2nd instar P.litura larvae, the ratio of LD 50 between PlNPV (LD 50 =16.02-53.53 PIB/Larva)and PlNP V+Bt(LD 50 =2.9-7.76 PIB/Larva ) is 5.5-6.9 while the ratio between PlNPV and PlNPV+Bt+AcMNPV A (LD 50 =3.16-15.469

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return