Different Nueleotide Sequences within One of Hepatitis B Virus Clones Patient

FANG De-Xin; GAN Ren-Bao; LI Zai-Beng; DI Chun-Sheng; ZHOU Zong-An

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February 1999

FANG De-Xin, GAN Ren-Bao, LI Zai-Beng, DI Chun-Sheng and ZHOU Zong-An. Different Nueleotide Sequences within One of Hepatitis B Virus Clones Patient[J]. Virologica Sinica, 1999, 14(1): 52-57.

Citation: FANG De-Xin, GAN Ren-Bao, LI Zai-Beng, DI Chun-Sheng, ZHOU Zong-An. Different Nueleotide Sequences within One of Hepatitis B Virus Clones Patient .VIROLOGICA SINICA, 1999, 14(1) : 52-57.

一例乙型肝炎患者体内不同HBV克隆的序列差异

1.
南京军区军事医学研究所,南京210002

摘要

采用ＰＣＲ扩增、噬菌体克隆和核苷酸序列分析的方法对一例乙型肝炎患者体内ＨＢＶ的ＨＢｓＡｇ编码区和部分前Ｓ２区进行序列分析。结果发现同一患者体内获得的不同ＨＢＶＤＮＡ克隆间存在个别碱基的差异，而这种差异并非方法学本身产生的。由此得出的结论是，这种碱基突变反映了乙型肝炎患者体内ＨＢＶ基因组实际存在的多态性。
- 乙型肝炎病毒
- , S基因
- , 序列分析
- , 点突变
- , 多态性

Different Nueleotide Sequences within One of Hepatitis B Virus Clones Patient

1.
Huadong Research Institute for Medical Biotechnics，Nanfing 210002

Abstract

The HBV HBsAg coding region of a hepatitis B patient was analyzed by PCR amplification, bacteriophage cloning and nucleotide sequencing. It was showed that among different clones from the same patient there were point mutations and these different nucleotide changes were not resulted from the methodologies. It is concluded that there are polymorphic HBV genomes within the same hepatitis B patient
- Hepatitis B virus
- , S gene
- , Nucleotide sequencing
- , Point mutation
- , Polymorphism

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Different Nueleotide Sequences within One of Hepatitis B Virus Clones Patient

1. Huadong Research Institute for Medical Biotechnics，Nanfing 210002

Abstract: The HBV HBsAg coding region of a hepatitis B patient was analyzed by PCR amplification, bacteriophage cloning and nucleotide sequencing. It was showed that among different clones from the same patient there were point mutations and these different nucleotide changes were not resulted from the methodologies. It is concluded that there are polymorphic HBV genomes within the same hepatitis B patient