Preparation of Indirect ELISA Reagent to Diagnose TORCH Infection

GONG Tian-Kui; HUANG He; JIA Lin; XIAO Gong-Yu; GONG Rui

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April 1999

GONG Tian-Kui, HUANG He, JIA Lin, XIAO Gong-Yu and GONG Rui. Preparation of Indirect ELISA Reagent to Diagnose TORCH Infection[J]. Virologica Sinica, 1999, 14(2): 123-128.

Citation: GONG Tian-Kui, HUANG He, JIA Lin, XIAO Gong-Yu, GONG Rui. Preparation of Indirect ELISA Reagent to Diagnose TORCH Infection .VIROLOGICA SINICA, 1999, 14(2) : 123-128.

ToRCH系列酶标诊断试剂的研制

1.
湖北省医学科学院病毒研究所．武汉430079

摘要

报道了用辣根过氧化物酶标记的抗人ＩｇＧ和抗人ＩｇＭ（ｕ链）单克隆抗体作第二抗体，用自己培养、纯化的弓形体（Ｔｏ）、风疹病毒（ＲｕＶ）、巨细胞病毒（ＣＭＶ）和单纯疱疹病毒（ＨＳＶ１２）的虫体和病毒抗原包被酶标板，研制出检测ＴｏＲＣＨ系列的特异性ＩｇＧ和ＩｇＭ的间接ＥＬＩＳＡ试剂。质量检定结果表明，该试剂特异性强、本底低，能有效消除ＲＦ因子等干扰因素的影响；灵敏度达１∶１６０～６４０；精密性好，变异系数（Ｃ．Ｖ）在１．４％～９．０％；试剂稳定，３７℃存放４ｄ，各项指标的变化率不超过１５％。
- ToRCH感染
- , 间接ELISA
- , 诊断试剂

Preparation of Indirect ELISA Reagent to Diagnose TORCH Infection

1.
tnggigute of Virology．HubeiAcademyof Medical Science，Wuhan 430079

Abstract

Torch is the abbreviation of four pathgenic microbols: Tonoplasma gondic (Toxo), Rubella Virus (RuV), Cytomegalovirus (CMV), and Herpes Simplex Virus (HSV 1 2 ). A new indirect ELISA reagent to detect the specific IgG and IgM antibody to ToRCH has been prepared. McAbs, labeled with horseradish peroxidase, against human IgG and IgM are used as the second antibodies. Purified Toxo, RuV, CMV, HSV 1 2 are used as antigens to coat wells. The reagent's quality has been tested. The result showed that the reagent has high specificity and low background. The sensitivity is up to 1∶160～640, the precision is high and the coefficient of variation (C.V) is between 1.4%～9.0%. The reagent is stable (stored at 37 ℃ for 4 days, the change range of each target is no more than 15%).
- TORCH infection
- , Indirect ELISA
- , Diagno~ reagent

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Preparation of Indirect ELISA Reagent to Diagnose TORCH Infection

1. tnggigute of Virology．HubeiAcademyof Medical Science，Wuhan 430079

Abstract: Torch is the abbreviation of four pathgenic microbols: Tonoplasma gondic (Toxo), Rubella Virus (RuV), Cytomegalovirus (CMV), and Herpes Simplex Virus (HSV 1 2 ). A new indirect ELISA reagent to detect the specific IgG and IgM antibody to ToRCH has been prepared. McAbs, labeled with horseradish peroxidase, against human IgG and IgM are used as the second antibodies. Purified Toxo, RuV, CMV, HSV 1 2 are used as antigens to coat wells. The reagent's quality has been tested. The result showed that the reagent has high specificity and low background. The sensitivity is up to 1∶160～640, the precision is high and the coefficient of variation (C.V) is between 1.4%～9.0%. The reagent is stable (stored at 37 ℃ for 4 days, the change range of each target is no more than 15%).