Cloning and Sequencing of Envelope Glycoprotein E0 Gene of Hog Cholera Virus Strain C

LI Gong-Wei; LIU Xiang-Chao; LI Xiao-Bing; HAN Xue-Qing; YAN Shen

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April 1999

LI Gong-Wei, LIU Xiang-Chao, LI Xiao-Bing, HAN Xue-Qing and YAN Shen. Cloning and Sequencing of Envelope Glycoprotein E0 Gene of Hog Cholera Virus Strain C[J]. Virologica Sinica, 1999, 14(2): 169-173.

Citation: LI Gong-Wei, LIU Xiang-Chao, LI Xiao-Bing, HAN Xue-Qing, YAN Shen. Cloning and Sequencing of Envelope Glycoprotein E0 Gene of Hog Cholera Virus Strain C .VIROLOGICA SINICA, 1999, 14(2) : 169-173.

我国猪瘟病毒兔化弱毒株囊膜糖蛋白E0基因的克隆及序列测定

1.
长春农牧大学,长春130062
2.
兰州兽医研究所,兰州730046

摘要

采用异硫氰酸胍一步法从４８０代猪瘟病毒兔化弱毒株（ＨＣＬＶ）脾毒中提取总ＲＮＡ，以该ＲＮＡ为模板，进行反转录，然后采用套式ＰＣＲ扩增出ＨＣＬＶ的囊膜糖蛋白Ｅ０基因，琼脂糖凝胶电泳表明其大小与预计相符。将扩增出的Ｅ０基因克隆到ｐＧＥＭＴ载体中，用自动序列分析仪对其进行序列测定。将测得的序列及推导的氨基酸序列与国外测得的Ｃ株相应序列进行比较，结果发现，它们之间核苷酸序列同源性为９９．０８％，氨基酸序列同源性为９８．４２％。
- 猪瘟病毒兔化弱毒株
- , 囊膜糖蛋白E0基因
- , 克隆
- , 序列测定

Cloning and Sequencing of Envelope Glycoprotein E0 Gene of Hog Cholera Virus Strain C

1.
ChangchunUniversity ofAgricultural andAnimal Sciences,Changchun 130062
2.
Lanzhou Veterinary Institute，Lanzhou 730046

Abstract

The envelope glycoprotein E0 gene of hog cholera virus (HCV) strain C was amplified from total RNA extracted from HCV strain C infected rabbit spleen by reverse transcription and nested PCR. The PCR product was cloned into pGEM T vector. Nucleotide sequencing was performed using an ABI PRISM sequencing device. Based on the incorporation of fluorescence labelled dideoxynuclotide teminators, the sequence was compared with HCV strain C sequenced by Moormann et al . The result showed that their homologies on nucleotide and amino acid sequences were 99.08% and 98.42%, respectively.
- Hog cholera virus strain C
- , Envelope glycoprotein E0 gene
- , a oning
- , Sequencing

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning and Sequencing of Envelope Glycoprotein E0 Gene of Hog Cholera Virus Strain C

1. ChangchunUniversity ofAgricultural andAnimal Sciences,Changchun 130062
2. Lanzhou Veterinary Institute，Lanzhou 730046

Abstract: The envelope glycoprotein E0 gene of hog cholera virus (HCV) strain C was amplified from total RNA extracted from HCV strain C infected rabbit spleen by reverse transcription and nested PCR. The PCR product was cloned into pGEM T vector. Nucleotide sequencing was performed using an ABI PRISM sequencing device. Based on the incorporation of fluorescence labelled dideoxynuclotide teminators, the sequence was compared with HCV strain C sequenced by Moormann et al . The result showed that their homologies on nucleotide and amino acid sequences were 99.08% and 98.42%, respectively.