酵母菌ＳＨ２发酵产物（简称ＦＳＨ２），用ＳｅｐｈａｄｅｘＧ７５凝胶层析和ＨＰＬＣ色谱层析分离纯化，收集到一组蛋白。该组蛋白在ＰＡＧＥ和ＳＤＳＰＡＧＥ电泳上带型一致，无蛋白亚基，分子量范围为５２～７２ｋＤ。凝胶上糖蛋白的特异性染色Ｓｃｈｉｆｆｓ染色显示阳性染色带；用Ｌｏｗｒｙｓ法测蛋白和硫酸酚法测糖，显示蛋白与糖的比例约为３∶１。该组蛋白与人干扰素作用，增强干扰素生物学效价分别为１．６～２．８倍和１．４～４．０倍；经ＳｅｐｈａｄｅｘＧ７５凝胶层析的ＦＳＨ２蛋白洗脱峰增效２．０１～５．６８倍；发酵液增效１．６４～６．８６倍。并证实酵母菌ＳＨ２增效干扰素的活性成分为５２～７２ｋＤ分子量范围的胞外糖蛋白（简称ＹＥＧＰｓ）。

The fermentation broth of yeast strain SH\-2?(FSH\-2) which could enhance the biological potency of human interferon alpha (huIFN ) was detected. Its enhancing ratio was 1.64～6.86 fold. A group of proteins was seperated and purified from the fermentation supernatant by chromatography on Sephadex G 75 and HPLC. Each protein showed the similar band on PAGE and SDS PAGE. Their molecular weights ranged from 52 to 72 kD. The proteins were stainded with periodic acid Schiff's agent. Lowry's method and sulfuric acid phenol method were respectively used to determine the contents of proteins and neutral sugars. The results showed the ratio of protein to sugar was 3∶1. These results indicated that the proteins were extracellular glycoproteins (YEGPs) secreted by yeast strain SH\-2. YEGPs could significantly enhance the biological potency of huIFN . It was veritied by the experiment of cytopathic effect inhibition in Wish cells. The enhancing ratios were 1.6～2.8 fold and 1.4～4.0 fold, respectively. The enhancing