Cloning and sequencing of the Buzura suppressaria nucleopolyhedrovirus DNA polymerase gene

GONG Min; LI Wei-Dong; LIANG Bu-Feng

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February 2000

GONG Min, LI Wei-Dong and LIANG Bu-Feng. Cloning and sequencing of the Buzura suppressaria nucleopolyhedrovirus DNA polymerase gene[J]. Virologica Sinica, 2000, 15(1).

Citation: GONG Min, LI Wei-Dong, LIANG Bu-Feng. Cloning and sequencing of the Buzura suppressaria nucleopolyhedrovirus DNA polymerase gene .VIROLOGICA SINICA, 2000, 15(1) : 59.

油桐尺蠖核型多角体病毒DNA聚合酶基因的克隆和序列分析

1.
中国科学院武汉病毒研究所

摘要

用PCR方法从油桐尺蠖核型多角体病毒 (BusuNPV)中扩增出DNA聚合酶基因片段 ,经pGEM T载体克隆到大肠杆菌DH5α菌株中。经自动序列分析仪测出DNA聚合酶基因 2 379bp长的核苷酸序列 ,推导出 793的氨基酸序列。氨基酸同源性比较显示 ,BusuNPV与HzSNPV的同源性最高 ,达 57% ;与OpMNPV的同源性最低 ,为 39.6 %。
- 油桐尺蠖核型多角体病毒 DNA聚合酶 PCR扩增序列分析

Cloning and sequencing of the Buzura suppressaria nucleopolyhedrovirus DNA polymerase gene

1.
Wuhan Institute of Virology,Academica Sinica

Abstract

The DNA polymerase gene of Buzura suppressaria nucleopolyhedrovirus (BusuNPV) was amplified by PCR using virus DNA template. The amplified fragment was cloned into E.coli via plasmid pGEM T. The fragment of insert was analyzed on an automated DNA sequencer. Its sequence showed that the insert has 2 379 nucleotides in length and 793 aa was predicated. Comparing with other six members of Baculoviridae , amaino acid sequence of the DNA polymerase of BusuNPV has 57% similarity with HzSNPV, and 39.6% with OpMNPV.
- Buzura suppressaria nucleopolyhedrovirus （BusuNPV） DNA polymerase PCR amplification Sequence analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning and sequencing of the Buzura suppressaria nucleopolyhedrovirus DNA polymerase gene

1. Wuhan Institute of Virology,Academica Sinica

Abstract: The DNA polymerase gene of Buzura suppressaria nucleopolyhedrovirus (BusuNPV) was amplified by PCR using virus DNA template. The amplified fragment was cloned into E.coli via plasmid pGEM T. The fragment of insert was analyzed on an automated DNA sequencer. Its sequence showed that the insert has 2 379 nucleotides in length and 793 aa was predicated. Comparing with other six members of Baculoviridae , amaino acid sequence of the DNA polymerase of BusuNPV has 57% similarity with HzSNPV, and 39.6% with OpMNPV.