以草鱼呼肠孤病毒(GCRV)感染的草鱼肾细胞系(CIK)为模型,进行了草鱼呼肠孤病毒在细胞内的形态发生的研究。当病毒以感染复数为5～10PFU/CELL感染CIK细胞时,在病毒感染细胞4h以内的切片中,可观察到脱去部分外层衣壳的不完整病毒颗粒。感染细胞8h,可观察到浆胞内病毒发生基质,其内含有大量的直径约50nm的亚病毒颗粒,无外层蛋白结构。感染12～16h后,这些亚病毒颗粒装配上外层蛋白结构,形成直径为72nm左右的成熟的病毒粒子。病毒感染细胞8h后,开始出现典型的病毒包含体,16～20h小时病毒包含体裂解,继而释放出有感染性的子代病毒颗粒。该结果有助于对GCRV致病机理的了解.

The morphogenesis of the Grass Carp Reovirus (GCRV) was studied in CIK cells. The CIK cells were infected by the GCRV at MOI (multiplicity of infection) of 5 10 PFU/Cell. It was demonstrated that the subviral particles without outer layer proteins could be seen in the cytoplasm at 4 hours post infection. At 8 hours post infection many viroplasms could be found in the cytoplasm of the cells. And there were many subviral particles without outer layer proteins in them. As the infection progressed, the mature virus, which is about 72 nm in diameter produced after the subviral particles were transiently enveloped during 12 16 hours post infection. During its replication, at 8 hour post infection inclusion bodies were formed, in which there were many immature and mature viruses. And the progeny virions were released after the inclusion bodies lysised at 16 20 hours post infection .