Study on RT-PCR／RFLP of Spike Protein S1 Gene of Avian Infectious Brochitis Virus H Strain

DIAO Zhen-Fen; HUANG E-Dong; WANG Lin-Chuan; LIU Gong-Beng; XIN Chao-An

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April 2000

DIAO Zhen-Fen, HUANG E-Dong, WANG Lin-Chuan, LIU Gong-Beng and XIN Chao-An. Study on RT-PCR／RFLP of Spike Protein S1 Gene of Avian Infectious Brochitis Virus H Strain[J]. Virologica Sinica, 2000, 15(2): 197-199.

Citation: DIAO Zhen-Fen, HUANG E-Dong, WANG Lin-Chuan, LIU Gong-Beng, XIN Chao-An. Study on RT-PCR／RFLP of Spike Protein S1 Gene of Avian Infectious Brochitis Virus H Strain .VIROLOGICA SINICA, 2000, 15(2) : 197-199.

鸡传染性支气管炎病毒H株纤突蛋白基因的RT-PCR/RFLP分型

1.
郑州牧业工程高等专科学拉,郑州450008

摘要

鸡传染性支气管炎病毒(ＩＢＶ)属于冠状病毒科冠状病毒属,可引起鸡呼吸道、输卵管、肾脏、肠道及腺胃等多部位病变。近年来,由于新的ＩＢＶ变异毒株不断出现,从而导致鸡传染性支气管炎病的不断爆发,造成严重的经济损失[1]。ＩＢＶ的基因组为单股ＲＮＡ,约27.6ｋｂ,该基因?..
- 鸡传染性支气管炎病毒
- , 纤突蛋白S1基因
- , 反转录和聚合酶链式反应

Study on RT-PCR／RFLP of Spike Protein S1 Gene of Avian Infectious Brochitis Virus H Strain

1.
College of Zhenzhou Animal Husbandry,Zhenzhou 450008，China

Abstract

A 1.66 kb expected S1 gene fragment of avian infectious brochitis virus (IBV) H strain was amplified by RT PCR. Its PCR/RFLP pattern was similar to IBV D 41 strain and M 41 strain when digested using BstYI, HaeⅢ and EcoRI respectively. IBV H strain was thought as Massachusetts serotype.
- Avian infectious bronchitis virus
- , S1 gene
- , RT-PCR/RFLP

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on RT-PCR／RFLP of Spike Protein S1 Gene of Avian Infectious Brochitis Virus H Strain

1. College of Zhenzhou Animal Husbandry,Zhenzhou 450008，China

Abstract: A 1.66 kb expected S1 gene fragment of avian infectious brochitis virus (IBV) H strain was amplified by RT PCR. Its PCR/RFLP pattern was similar to IBV D 41 strain and M 41 strain when digested using BstYI, HaeⅢ and EcoRI respectively. IBV H strain was thought as Massachusetts serotype.