Fusion Expression of Hepatitis C Virus Core Region and NS3 Region in E ．coli

LI Wei-Dong; LIANG Bu-Feng; QI Zi-Bai

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April 2000

LI Wei-Dong, LIANG Bu-Feng and QI Zi-Bai. Fusion Expression of Hepatitis C Virus Core Region and NS3 Region in E ．coli[J]. Virologica Sinica, 2000, 15(2): 204-207.

Citation: LI Wei-Dong, LIANG Bu-Feng, QI Zi-Bai. Fusion Expression of Hepatitis C Virus Core Region and NS3 Region in E ．coli .VIROLOGICA SINICA, 2000, 15(2) : 204-207.

丙型肝炎病毒Core与NS3基因的不同融合方式及其表达

1.
中国科学院武祝病毒研究所，武汉430071

摘要

丙型肝炎病毒(简称ＨＣＶ)是危害人类健康的传染性疾病,预防该病的传播主要借助于ＨＣＶ血清学诊断来筛选献血员和检测临床标本。从国内外已分离到的ＨＣＶ株得知,ＨＣＶ核心蛋白(Ｃｏｒｅ)和非结构蛋白ＮＳ3含有较强的优势抗原表位,其相应的抗体出现早,阳性率高,已成为目...
- 丙型肝炎病毒
- , Core基因
- , NS3基因
- , 基因融合
- , 表达

Fusion Expression of Hepatitis C Virus Core Region and NS3 Region in E ．coli

1.
Wuhan

Abstract

The gene encoding the core and NS3 proteins of hepatitis C virus was amplified by PCR, respectirely. Two genes were fused and formed the recombinant plamid pHCV CN (Core+NS3) and pHCV NC (NS3+Core). The fused plasmid were expressed in E.coli 06. The pHC CN plasmid expressed fussion protein was shown by a major band with a expected molecular weight about 68 kD on SDS PAGE. However, the pHCV NC plasmid expressed fussion protein was 66 kD in molecular weight. The results indicate that the pHCV NC fussion protein differs from the pHCV CN fussion protein in mulecular weight. It suggests that the fusion way is important for the structure of recombinant proteins.
- HCV
- , NS3 gene
- , Co re gene
- , Fusion protein
- , Expression

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Fusion Expression of Hepatitis C Virus Core Region and NS3 Region in E ．coli

1. Wuhan

Abstract: The gene encoding the core and NS3 proteins of hepatitis C virus was amplified by PCR, respectirely. Two genes were fused and formed the recombinant plamid pHCV CN (Core+NS3) and pHCV NC (NS3+Core). The fused plasmid were expressed in E.coli 06. The pHC CN plasmid expressed fussion protein was shown by a major band with a expected molecular weight about 68 kD on SDS PAGE. However, the pHCV NC plasmid expressed fussion protein was 66 kD in molecular weight. The results indicate that the pHCV NC fussion protein differs from the pHCV CN fussion protein in mulecular weight. It suggests that the fusion way is important for the structure of recombinant proteins.