Four Kinds of EBV-Antisense Oiigodeoxynucleotides Effect on Proliferation of EBV Containing SUNE-1 Cell Line

SHU Zhen-Yu; ZHOU Yuan-Beng; ZHANG Qing-Xiu; GAO Guo-Quan; DENG Yi-Qun; MA Jian-Quan

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June 2000

SHU Zhen-Yu, ZHOU Yuan-Beng, ZHANG Qing-Xiu, GAO Guo-Quan, DENG Yi-Qun and MA Jian-Quan. Four Kinds of EBV-Antisense Oiigodeoxynucleotides Effect on Proliferation of EBV Containing SUNE-1 Cell Line[J]. Virologica Sinica, 2000, 15(3): 209-213.

Citation: SHU Zhen-Yu, ZHOU Yuan-Beng, ZHANG Qing-Xiu, GAO Guo-Quan, DENG Yi-Qun, MA Jian-Quan. Four Kinds of EBV-Antisense Oiigodeoxynucleotides Effect on Proliferation of EBV Containing SUNE-1 Cell Line .VIROLOGICA SINICA, 2000, 15(3) : 209-213.

EB病毒4种反义寡核苷酸片段对鼻咽癌SUNE-1细胞株的影响

1.
中山医科大学生物化学教研室,广州510089

摘要

用不同浓度的EB病毒 4种 (BHRF1、DNA酶、核抗原 1及胸苷激酶 )反义寡核苷酸片段 ,(antisenseoligodeoxynucleotide ,ASO)分别与含 10 %胎牛血清或不含胎牛血清的人低分化鼻咽癌细胞株 (SUNE 1)共同培养 4 8h。结果仅适当浓度的BHRF1 ASO能引起无血清培养的SUNE 1细胞株增殖明显受抑制 ,透射电镜及琼脂糖凝胶电泳检测结果发现与SUNE 1细胞发生凋亡有关 ;而相同浓度的DNA酶、核抗原 1和胸苷激酶基因的ASO则无类似作用。
- EB病毒
- , 反义寡核苷酸
- , 细胞凋亡

Four Kinds of EBV-Antisense Oiigodeoxynucleotides Effect on Proliferation of EBV Containing SUNE-1 Cell Line

1.
Department of Bioclwmistry, Sun yat-sen

Abstract

Poorly differentiated nasopharyngeal carcinoma (SUNE 1) cell line was cultured with Epstein Barr virus (EBV) BHRF1, DNase, thymidine kinase (TK) and nuclear antigen 1 (EBNA1) antisense oligodeoxynucleotide (ASO) for 48 h respectively. Inhibited proliferation of SUNE 1 was only observed in the absence of fetal calf serum (FCS) and exposed to EBV BHRF1 ASO. The results from electron microscopy and agarose gel electrophoresis indicated that apoptosis only appeared in SUNE 1 cells exposed to BHRF1 ASO and absence of FCS. The other 3 kinds of EBV ASOs had not the similar effects at the same concentration.
- Epstein Barr virus
- , Antisense oligodeoxynucleotide
- , Apoptosis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Four Kinds of EBV-Antisense Oiigodeoxynucleotides Effect on Proliferation of EBV Containing SUNE-1 Cell Line

1. Department of Bioclwmistry, Sun yat-sen

Abstract: Poorly differentiated nasopharyngeal carcinoma (SUNE 1) cell line was cultured with Epstein Barr virus (EBV) BHRF1, DNase, thymidine kinase (TK) and nuclear antigen 1 (EBNA1) antisense oligodeoxynucleotide (ASO) for 48 h respectively. Inhibited proliferation of SUNE 1 was only observed in the absence of fetal calf serum (FCS) and exposed to EBV BHRF1 ASO. The results from electron microscopy and agarose gel electrophoresis indicated that apoptosis only appeared in SUNE 1 cells exposed to BHRF1 ASO and absence of FCS. The other 3 kinds of EBV ASOs had not the similar effects at the same concentration.