研究了人类疱疹病毒 7型 (HHV 7)南京株YY5在脐血单个核细胞 (CBMCs)及SUPT1细胞株上生长特点。观察病毒感染细胞后不同时间病变效应程度 ,记数死亡细胞百分率 ,间接免疫荧光染色记数抗原表达阳性细胞数 ,并用透射电镜观察病毒感染细胞超微结构变化及病毒复制不同时期的特点。结果发现 :HHV 7在CBMCs及SUPT1上出现CPE时间迟于HHV 6,且CPE程度也低于HHV 6;HHV 7在SUPT1细胞上尽管有CPE及抗原表达 ,但很难找到成熟的病毒颗粒 ;电镜下 ,病毒主要存在于肿胀的病变细胞内 ,且病毒感染细胞常出现核染色质聚集 ,核固缩 ,及胞浆细胞器空泡化 ,细胞裂解等特点 ;成熟的HHV 7颗粒直径约 170 - 190nm ,核衣壳约 90 - 10 0nm ,核衣壳内致密核心约 4 0nm ,核衣壳与包膜之间是丰富的被膜约 30 - 35nm ,包膜上有刺突。HHV 7常发现无核心的病毒颗粒。

The growth characteristics of Human herpesvirus 7 local strain YY5 were investigated in cord blood mononuclear cells (CBMCs) and CD4 + lymphoblastic cell line SUPT1. Virus growth was demonstrated by cytopathic effect (CPE), percentage of dead cells count, indirect immunofluorescence test with viral antigen expression at different days post infection. At the same time, ultrastructural appearance of infected cells and of HHV 7 in various period were investigated by transmission electron microscopy. The results are as follows: (1) The CPE peak time of HHV 7 was latter than that of HHV 6, and CPE of HHV 7 was less pronounced. (2) Although the CPE was obvious and the viral antigen was found in SUPT1 cells, mature virus particles were hardly observed intra and extra these cells. (3) The virions mainly laid in the giant syncytia. HHV 7 infected cells exhibited heterochromatin clumping and margination or pyknosis, vacuolized cytoplasm, discontinuation of the plasma membrane and necrosis. (4) Mature virus parti