HUANG Can-hua, SHI Zheng, ZHANG Lu, XIE Yun-li, ZHANG Li, CHEN Di and wU Qing-jiang. Homology Comparison of White Spot Syndrome Baculovirus(WSSV) from Penaeid shrimp[J]. Virologica Sinica, 2001, 16(1): 81-84.
Citation:
HUANG Can-hua, SHI Zheng, ZHANG Lu, XIE Yun-li, ZHANG Li, CHEN Di, wU Qing-jiang.
Homology Comparison of White Spot Syndrome Baculovirus(WSSV) from Penaeid shrimp .VIROLOGICA SINICA, 2001, 16(1)
: 81-84.
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摘要
比较我国沿海不删海域对虾白斑综合征杆状病毒三十分离株:即唐海分离株(渤海湾).宁渡分离株(东海). 豫圳分离株(南海)的同 性 三十wsSv分离株基因组的限制性内切酶(Sac l,H/ndIll,Pst I)酶切多态(RFI P) 以及病毒结构蛋白图谱先全一致.证实造成我国从南至北对虾爆发性流行病的对虾白斑杆状病毒为同一种病毒 利用高保真Taq酶.分别以针i道的日本对虾扦状病毒(RV—PJ=PRDV).斑节对虾白斑综合征杆状病毒(WSBV= PmNOBII1)基因组核酸 段特异性引物进行PCR扩增.结果均能从中国耐虾白斑杆状病毒(WSSV)基因组中扩增 得到相应大小的I:K~R产物.扩增产物序列分析表明中国对虾白斑杆状病毒(wssv)与斑节对虾白斑综合征杆状病 毒(WSBV=PmNOBIII).1l丰对虾扦状病毒(RV—PJ:PRDV)同源率分别为100%与97%.其结果为证实亚洲及 太平洋地区对虾自斑综舟 }r状病毒为同一种病毒或同一种病毒的不同株系提供了证据。
关键词:
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比较我国沿海不删海域对虾白斑综合征杆状病毒三十分离株:即唐海分离株(渤海湾).宁渡分离株(东海).
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豫圳分离株(南海)的同
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性
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三十wsSv分离株基因组的限制性内切酶(Sac
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l
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H/ndIll
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Pst
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I)酶切多态(RFI
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P)
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以及病毒结构蛋白图谱先全一致.证实造成我国从南至北对虾爆发性流行病的对虾白斑杆状病毒为同一种病毒
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,
利用高保真Ta
Homology Comparison of White Spot Syndrome Baculovirus(WSSV) from Penaeid shrimp
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1.
1 State Key Laboratory ofFresh,raterEcology andB~otechnology.1nstituteof Hydrohiology,Academia Sinica, Wuhan 430072.China
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2.
Jointtaboratot~+of InvetebrateVirology.WuhanInstitute VirologyAcademia Sinica,Wuhan 43007I.China
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Abstract
Homology of three WSSV isolates.which were sampled from representative maritime space of China:Tanghai isolate(13o Bay of China),Ningbo isolate(East China Sea).Shenzhen isolate (South China Sea)was compared.Both of the genome RFLP patterns and the characteristic structural proteins SDS-PAGE electropherograms showed that they were quite$a/’ae.It suggested that they were the same kind of WSSV virus that caused explosive epidemic diseases of shrimps(EEDs)throughout southern and northern China The$drne large PCR products achieved when using the PCR primers from RV—PJ:PRDV (P japonicus.Japan)and WSBV=PmNOBIII(P.morR~don Taiwan,Chi— na)respectively to amplify the genome from P.chinensis(Tanghai,China)with high fidelity Taq Polymerase.The sequence identities of WSSV from P.chinens~ with those from RV—PJ=PRDV (P 血ponicus,Japan)and WSBV=PmNOBill(P.monodon Taiwan.China)are 97% and 100% respectively,the results provided additiona l evidence that W SSV reported in different parts of the Asian and Pacific regions maybe quite the same or jLISt different variants of the same virus
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References
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Proportional views
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