HUANG Wen— ZHANG Yu-zeng.WANG Jing—lin.ZHANG Hal lin, The Studies on the Host Cell Apoptosis of Yunnan Sindbis Virus[J]. Virologica Sinica, 2002, 17(2): 110-113.
Citation:
HUANG Wen— ZHANG Yu-zeng.WANG Jing—lin.ZHANG Hal lin, .
The Studies on the Host Cell Apoptosis of Yunnan Sindbis Virus .VIROLOGICA SINICA, 2002, 17(2)
: 110-113.
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摘要
为了了解云南辛德毕斯病毒在BHK21细胞上的生长特性 并观察该病毒在BHK21细胞、3d龄和2周龄小 白鼠中是否有嗣亡反应出现。在接种后不同时间取样,检测病毒的滴度,电镜捡测 亡细胞,检测细胞的DNA阶 梯。一步生长曲线结果表明病毒在出现CPE之前就有大量繁殖。秋水仙索对该病毒的成熟释放没有抑制作用。 该病毒对BHK21、小白鼠均可产生细胞i崩亡。
The Studies on the Host Cell Apoptosis of Yunnan Sindbis Virus
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Abstract
The one step growth curve of YN87448 strain virus in BHK21 cell showed that a lot of progeny viruses could be observed at 16 hr.after infection The tiler of virus reached the highest about 10一 0TCDS0 at 48 hour post infection.The titer of virus is not reduced by Co1.The apo ptosis was observed by the electron m icroscope and the DNA ladder was detected by agarose gel eletrophoresis. YN87448 strain virus can cause apo tosis in BHK21 cell and mice
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References
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Proportional views
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