HCV Core Protein Induces Apoptosis in Cos-7 Cell Line

SHE Ying—long; YE Lin—bai; LIAO Qing-jiao; YE Li; GUO Tai—lin; YANG Xiao-jun

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August 2003

SHE Ying—long, YE Lin—bai, LIAO Qing-jiao, YE Li, GUO Tai—lin and YANG Xiao-jun. HCV Core Protein Induces Apoptosis in Cos-7 Cell Line[J]. Virologica Sinica, 2003, 18(4): 326-329.

Citation: SHE Ying—long, YE Lin—bai, LIAO Qing-jiao, YE Li, GUO Tai—lin, YANG Xiao-jun. HCV Core Protein Induces Apoptosis in Cos-7 Cell Line .VIROLOGICA SINICA, 2003, 18(4) : 326-329.

HCV核心蛋白诱导Cos一7细胞凋亡

1.
武汉大学生命科学学院病毒研究所，湖北武汉，430072

摘要

将丙型肝炎病毒(HCV)核心蛋白(Core)基因的全长序列插入到真核表达载体pCDNA3 CMV启动子下游，构建真核表达载体pCDNA3．Core，用脂质体LipoVec 。M转染Cos一7细胞系进行瞬时表达；DNA转染24h后用免疫斑点试验检测在细胞中表达的Core蛋白；转染72h后用Hoechst染色和DNA Ladder检测Cos一7细胞的凋亡情况；荧光染色观察到了细胞凋亡核碎裂，琼脂糖凝胶电泳也呈现出180—200bp整数倍的梯形带，呈现典型的细胞凋亡特征。这些结果表明HCV Core蛋白的表达能引起Cos一7细胞凋亡，Core蛋白的这种功能可能在HCV 的持续感染过程中起着一定的作用。
- 丙型肝炎病毒
- , 核心蛋白
- , 细咆凋亡

HCV Core Protein Induces Apoptosis in Cos-7 Cell Line

1.
Institute of virology,Wuhan University,Wuhan 430072，China

Abstract

Abstract：Recombinant plasmid pCDNA3一Core was constructed by inserting Hepatitis C virus(HCV一1 1 core gene into the site between EcoR I and Xba I of eukaryotic expression vector pCDNA3．The recombinan t plasmid pCDNA3一Core was tran sfected to COS一7 cellline by LipoVec ．Expression of core protein was detected by immunology Dot blot 24h after transfection．Th e nuclear cracks in tran sfected cell was observed by Hoechst 3325 8 staining and the 1 80—200bp DNA ladders alSO was detected 72h after transfection．Th ese results reveal that HCV Core protein Can induce apoptosis of COS一7 cell and its function nlay play an importan t role in HCV chronic and persistan t infection．
- Hepatitis C virus(HCV)
- , Core protein
- , Apoptosis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

HCV Core Protein Induces Apoptosis in Cos-7 Cell Line

1. Institute of virology,Wuhan University,Wuhan 430072，China

Abstract: Abstract：Recombinant plasmid pCDNA3一Core was constructed by inserting Hepatitis C virus(HCV一1 1 core gene into the site between EcoR I and Xba I of eukaryotic expression vector pCDNA3．The recombinan t plasmid pCDNA3一Core was tran sfected to COS一7 cellline by LipoVec ．Expression of core protein was detected by immunology Dot blot 24h after transfection．Th e nuclear cracks in tran sfected cell was observed by Hoechst 3325 8 staining and the 1 80—200bp DNA ladders alSO was detected 72h after transfection．Th ese results reveal that HCV Core protein Can induce apoptosis of COS一7 cell and its function nlay play an importan t role in HCV chronic and persistan t infection．