Comparative Analysis of Genomic Organization of the 5 and of Four Strain SARS—口ssociated Coronaviruses

CHANG Guo—hui; PENG W en—ming; LIU Bo—hua; FAN Bao—chang; LIU Hong; DENG Yong—qiang; LU Fu—shuang; YANG Bao—an; QIN E-de; ZHU Qing—yu*

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August 2003

CHANG Guo—hui, PENG W en—ming, LIU Bo—hua, FAN Bao—chang, LIU Hong, DENG Yong—qiang, LU Fu—shuang, YANG Bao—an, QIN E-de and ZHU Qing—yu*. Comparative Analysis of Genomic Organization of the 5 and of Four Strain SARS—口ssociated Coronaviruses[J]. Virologica Sinica, 2003, 18(4): 330-334.

Citation: CHANG Guo—hui, PENG W en—ming, LIU Bo—hua, FAN Bao—chang, LIU Hong, DENG Yong—qiang, LU Fu—shuang, YANG Bao—an, QIN E-de, ZHU Qing—yu*. Comparative Analysis of Genomic Organization of the 5 and of Four Strain SARS—口ssociated Coronaviruses .VIROLOGICA SINICA, 2003, 18(4) : 330-334.

4株SARS冠状病毒基因组5 端序列的分析与比较

1.
军事医学科学院微生物流行病研究所，北京，100071

摘要

摘要：利用5 RACE 试剂盒对从中国不同地区、不同SARS患者体中分离的SARS．CoV基因组5 端序列进行 RT．PCR扩增，并将扩增产物克隆至T easy vector。扩增片段的序列测定结果表明：所分离的4株SARS．CoV基因组5 端非编码区的核苷酸序列和其他国家和地区报道的序列基本一致，而且所形成二级结构也完全相同，但与已知普通冠状病毒的差别较大。同时发现在依赖于RNA的RNA聚合酶起始密码子上游一197 nt处有冠状病毒典型的转录调控核心保守序列5 -CUAAAC-3
- SARS冠状病毒(SARS．CoV)
- , 基因组5 端结构
- , 序列分析
- , 转录调控序列SARS冠状病毒(SARS．CoV)
- , 基因组5 端结构
- , 序列分析
- , 转录调控序列

Comparative Analysis of Genomic Organization of the 5 and of Four Strain SARS—口ssociated Coronaviruses

1.
Institute of Microbiology and epidemiology,Academy ofMilitary Medical Sciences，Beijing 100071，China J

Abstract

Abstract：The viral RNAs were isolated from the infected Vero—E6 cells．then the templates were produced by reverse tran scription reaction．Two pairs of primers were used to amplify the 5 一ends of four strains of SAR． ssociated coronavirus(SARS—CoV)by RACE．cDNA fragments with the length of about 420 bp were amplified．The fragments were then purified an d sequenced．The sequences of 5 一UTRs of SARS —CoV isolated in China were the same as those isolated in other countries an d regions． such as Tor2 strain，Urbani strain，HKU—Su l 0 strain，CUHK—W l strain，S 2500 strain an d SIN2677 strain．Th e secondary structures formed by 5 一UTRs of all known SARS—CoV were almost identica1．but significan tly diferent from other kn own non—SARS coronaviruses．Sequence an alysis indicated that the conserved core sequence(5 一CUAAAC一3 1 of transcription regulating sequence in some coronaviruses was about—l 97 nt upstream from the start codon．
- SARS-associated coronavirus(SARS—CoV)
- , 5 一ends of genomic organization
- , Sequence analysis
- , Tran scription regulating sequence

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Comparative Analysis of Genomic Organization of the 5 and of Four Strain SARS—口ssociated Coronaviruses

1. Institute of Microbiology and epidemiology,Academy ofMilitary Medical Sciences，Beijing 100071，China J

Abstract: Abstract：The viral RNAs were isolated from the infected Vero—E6 cells．then the templates were produced by reverse tran scription reaction．Two pairs of primers were used to amplify the 5 一ends of four strains of SAR． ssociated coronavirus(SARS—CoV)by RACE．cDNA fragments with the length of about 420 bp were amplified．The fragments were then purified an d sequenced．The sequences of 5 一UTRs of SARS —CoV isolated in China were the same as those isolated in other countries an d regions． such as Tor2 strain，Urbani strain，HKU—Su l 0 strain，CUHK—W l strain，S 2500 strain an d SIN2677 strain．Th e secondary structures formed by 5 一UTRs of all known SARS—CoV were almost identica1．but significan tly diferent from other kn own non—SARS coronaviruses．Sequence an alysis indicated that the conserved core sequence(5 一CUAAAC一3 1 of transcription regulating sequence in some coronaviruses was about—l 97 nt upstream from the start codon．