XIE Yun—li, ZHANG Shu—yong, HUANG Ru and SHI Zheng—li*. A Modified Technique for Purifying White spot syndrome virus[J]. Virologica Sinica, 2003, 18(4): 391-393.
Citation:
XIE Yun—li, ZHANG Shu—yong, HUANG Ru, SHI Zheng—li*.
A Modified Technique for Purifying White spot syndrome virus .VIROLOGICA SINICA, 2003, 18(4)
: 391-393.
-
摘要
对虾白斑综合征病毒(White spot syndrome virus，wSSV)是对虾养殖业的主要病原， 自1992 年以来一直严重影响对虾的产量和质量，造成巨大 的经济损失。由于其宿主范围涉及几乎所有的水生 甲壳类动物，因此成为水生动物病毒研究领域重要 的病毒之一。在对虾白斑综合征病毒研究的过程 中，病毒的提纯是关键的一步。由于该病毒的囊膜 蛋白很容易降解，很难得到完整的病毒粒子。传统 的蔗糖密度梯度离心介质不仅会破坏病毒囊膜，而 且还会丢失大量的病毒粒子，从而不易得到大量 的、完整的病毒粒子[1]。曾经报道一些改良的提纯 技术可以得到纯度较高的核衣壳和病毒粒子， 为 WSSV 基因组研究奠定了良好的基础[2,3]。本论文 报道的是一种改良的、能大量获得完整病毒粒子的 蔗糖密度梯度提纯技术。
A Modified Technique for Purifying White spot syndrome virus
-
1.
Key laboratory of Molecular Virology,Joint·Laboratory of Invertebrate Hrology,Wuhan Institute of Hrology,Chinese Academy of Sciences，Wuhan 430071，China
-
Abstract
Abstract：The envelope proteins of White spot syndrome virus(WSSV)are very fragile and easy to be destroyed during purification．It was difi cult to obtain a large quan tity of intact virions by routine sucrose gradient centrifugation．After modifying the sucrose gradient by adding citrate sodium ，we can obtain a large quantity of intact virions and nucleocapsids．Th is purified virions and nucleocapsids were subsequently used for an alyzing viral structural proteins an d DNA extraction．Th e result showed that this modified technique is very efi cient for virus purification．
-
-
References
-
Proportional views
-
-
DownLoad: