A Backflushing Ultrafiltration Technique for Concentrating Cyanophage

LUO Wen—qing; JU Chuan; CHENG Kai; ZHAO Yi-jun ”; SHI Zheng—li

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August 2003

LUO Wen—qing, JU Chuan, CHENG Kai, ZHAO Yi-jun ” and SHI Zheng—li. A Backflushing Ultrafiltration Technique for Concentrating Cyanophage[J]. Virologica Sinica, 2003, 18(4): 397-400.

Citation: LUO Wen—qing, JU Chuan, CHENG Kai, ZHAO Yi-jun ”, SHI Zheng—li. A Backflushing Ultrafiltration Technique for Concentrating Cyanophage .VIROLOGICA SINICA, 2003, 18(4) : 397-400.

一种浓缩噬藻体的反冲超滤技术

1.
1．华中师范大学生命科学学院，湖北武汉，430079：2．中国科学院武汉病毒研究所无脊椎动物病毒学联合开放实验室，湖北武汉，430071

摘要

噬藻体(Cyanophage)是一类感染蓝藻的病毒，形态上同于噬菌体I ，近期的研究表明，噬藻体作为水体环境中活跃的动态因子，在控制水体初级生产力和有害藻类水华(Harmful Algal Bloom，HAB) 方面可能发挥着重要的作用【2’引，甚至影响水体生态系统中食物链的结构【4】，因此研究水体中噬藻体的生理生态学特性对于了解其生态功能是非常重要的，但是由于自然水体中的噬藻体浓度往往较低，难以直接对其进行定性或定量研究，所以对天然水样中的噬藻体进行高效、快速的浓缩是研究噬藻体生态地位和功能的关键和难点。超滤法是国际上流行的浓缩自然水样中病毒的方法，引，但在我国尚未见报道。基于超滤的原理，本实验室对超滤法进行了改进，建立了一套能够有效浓缩水样中噬藻体的方法⋯反冲超滤法，此法浓缩工艺简单，耗时短，费用低，效率高。
- 超滤
- , 反冲
- , 噬藻体
- , 浓缩

A Backflushing Ultrafiltration Technique for Concentrating Cyanophage

1.
College ofLife Science，Central China Normal University,Wuhan 430079,China

Abstract

Abastract：Most of the cyanophages were adsorbed onto the ultrafilter membrance when pre—filtrated water sample passed through the tangential flow ultrafiltration system．In order to recover much more cyanophages，we used a technique named‘‘backflushing ultrafiltration”．The results indicated that when the volume of the backflush liquid was raised step by step，the concentration multiple of the cyanophage would gradually decrease，while the recovery rate of the cyanophage would gradually increase．In our performance，the maximal concentration multiple an d the recovery rate of cyan ophage could reach up to 30 times an d more than 90％ severally．Th is method was tested to be efective for concentrating cyan ophage from large volume liquid sample．M oreover，the concertration multiple of the cyan ophage could be elevated to nearly one—hundred times when two—step backflushing ultrafiltration was adopted．
- Ultrafiltration
- , Backflush
- , Cyan ophage
- , Concentration

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

A Backflushing Ultrafiltration Technique for Concentrating Cyanophage

1. College ofLife Science，Central China Normal University,Wuhan 430079,China

Abstract: Abastract：Most of the cyanophages were adsorbed onto the ultrafilter membrance when pre—filtrated water sample passed through the tangential flow ultrafiltration system．In order to recover much more cyanophages，we used a technique named‘‘backflushing ultrafiltration”．The results indicated that when the volume of the backflush liquid was raised step by step，the concentration multiple of the cyanophage would gradually decrease，while the recovery rate of the cyanophage would gradually increase．In our performance，the maximal concentration multiple an d the recovery rate of cyan ophage could reach up to 30 times an d more than 90％ severally．Th is method was tested to be efective for concentrating cyan ophage from large volume liquid sample．M oreover，the concertration multiple of the cyan ophage could be elevated to nearly one—hundred times when two—step backflushing ultrafiltration was adopted．