Establishment of Nested RT-PCR Diagnostic Method for Porcine Epidemic Diarrhea Virus

ZHANG Su-fang; JIA Yun; WANG Min-xiu; NI Yan-xiu; HE Kong-wang; CHEN Pu-yan

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April 2004

ZHANG Su-fang, JIA Yun, WANG Min-xiu, NI Yan-xiu, HE Kong-wang and CHEN Pu-yan. Establishment of Nested RT-PCR Diagnostic Method for Porcine Epidemic Diarrhea Virus[J]. Virologica Sinica, 2004, 19(2): 174-176.

Citation: ZHANG Su-fang, JIA Yun, WANG Min-xiu, NI Yan-xiu, HE Kong-wang, CHEN Pu-yan. Establishment of Nested RT-PCR Diagnostic Method for Porcine Epidemic Diarrhea Virus .VIROLOGICA SINICA, 2004, 19(2) : 174-176.

猪流行性腹泻病毒嵌套式RT-PCR检测方法的建立

张素芳 ^1,3 ,
王敏秀 ² ,
倪艳秀 ¹ ,
何孔旺 ³ ,
陈溥言 ^3*,, ,

1.
1. 南京农业大学农业部动物疫病诊断与免疫重点开放实验室，江苏南京 210095
2.
沈阳农业大学动物医学院，辽宁沈阳 110161
3.
江苏省农业科学院农业部畜禽疫病诊断重点开放实验室，江苏南京 210014

通讯作者： 陈溥言, ;

摘要
- 猪流行性腹泻病毒
- , 嵌套式PCR
- , 酶切鉴定

Establishment of Nested RT-PCR Diagnostic Method for Porcine Epidemic Diarrhea Virus

1.
1. Key Laboratory of Animal Disease diagnosis and Immunology, Ministry of Agriculture at Nanjing Agricultural University, Nanjing 210095
2.
Department of veterinary medicine, Shenyang Agricultural University, Shenyang 110161
3.
Key Lab of Animal and Poultry Diseases Diagnostic, Ministry of Agriculture at jiangsu Academy of Agricultural Science, Nanjing, 210014

Corresponding author: HE Kong-wang,

Abstract

Two pairs of primers were designed according to the N gene sequence of PEDV-CV777 strain in Genebank (No. AF353511). PCR amplification product of outer-primers was 1328bp, and the inter-primers amplification product was 538bp. With the primers, a nested PCR assay was established to detect PEDV. This method was sensitive and specific and could be used in PEDV diagnosis and epidemiological investigation.
- Porcine epidemic diarrhea virus
- , Nested-PCR
- , Identification by restriction analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Establishment of Nested RT-PCR Diagnostic Method for Porcine Epidemic Diarrhea Virus

Corresponding author: HE Kong-wang,

1. 1. Key Laboratory of Animal Disease diagnosis and Immunology, Ministry of Agriculture at Nanjing Agricultural University, Nanjing 210095
2. Department of veterinary medicine, Shenyang Agricultural University, Shenyang 110161
3. Key Lab of Animal and Poultry Diseases Diagnostic, Ministry of Agriculture at jiangsu Academy of Agricultural Science, Nanjing, 210014

Abstract: Two pairs of primers were designed according to the N gene sequence of PEDV-CV777 strain in Genebank (No. AF353511). PCR amplification product of outer-primers was 1328bp, and the inter-primers amplification product was 538bp. With the primers, a nested PCR assay was established to detect PEDV. This method was sensitive and specific and could be used in PEDV diagnosis and epidemiological investigation.