Low-temperature Induced Expression of HIV gp41 Gene Segments

YUAN Yu-hua; BI Chang-hao; LI Ju; WANG Xue-qian; GENG Yun-qi; CHENG Qi-min

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April 2004

YUAN Yu-hua, BI Chang-hao, LI Ju, WANG Xue-qian, GENG Yun-qi and CHENG Qi-min. Low-temperature Induced Expression of HIV gp41 Gene Segments[J]. Virologica Sinica, 2004, 19(2): 179-181.

Citation: YUAN Yu-hua, BI Chang-hao, LI Ju, WANG Xue-qian, GENG Yun-qi, CHENG Qi-min. Low-temperature Induced Expression of HIV gp41 Gene Segments .VIROLOGICA SINICA, 2004, 19(2) : 179-181.

HIV gp41基因分段低温诱导表达*

1.
南开大学生命科学学院，天津 300071

通讯作者： 陈启民*,

摘要
- HIV gp41
- , 低温诱导
- , 毒性

Low-temperature Induced Expression of HIV gp41 Gene Segments

1.
College of Life Science, Nankai University, Tianjin 300071,China

Corresponding author: CHENG Qi-min,

Abstract

Clone N3 and C from Human immunodeficiency virus(HIV) gp41 gene were expressed using the pET expression system. When induced by IPTG at 37℃, both two clones did not express in E.coli BL21(DE)3. Howerver, when induced at 16℃, the two clones were both overexpressed, and the amount of the product was about 20% of the total bacteria protein. In Western blotting test, the protein product could react with HIV-positive serum. After IPTG induction, E. coli cells had much higher death rate at 37℃ than at 16℃; [3H]uridine release assay also showed that after IPTG induction, E. coli had a higher release at 37℃. The results suggested that overexpression of the two proteins was due to their decreased toxicity at lower temperature.
- Human immunodeficiency virus (HIV) gp41 gene
- , Low temperature induction
- , Toxic

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Low-temperature Induced Expression of HIV gp41 Gene Segments

Corresponding author: CHENG Qi-min,

1. College of Life Science, Nankai University, Tianjin 300071,China

Abstract: Clone N3 and C from Human immunodeficiency virus(HIV) gp41 gene were expressed using the pET expression system. When induced by IPTG at 37℃, both two clones did not express in E.coli BL21(DE)3. Howerver, when induced at 16℃, the two clones were both overexpressed, and the amount of the product was about 20% of the total bacteria protein. In Western blotting test, the protein product could react with HIV-positive serum. After IPTG induction, E. coli cells had much higher death rate at 37℃ than at 16℃; [3H]uridine release assay also showed that after IPTG induction, E. coli had a higher release at 37℃. The results suggested that overexpression of the two proteins was due to their decreased toxicity at lower temperature.