Isolation and Identification of an Avian Influenza Virus of Subtype H5N1 from Hubei Province

KOU Zheng; TANG Li-jun; LEI Fu-min; AN Xue-fang; FAN Zhao-jun; LI Tian-xian

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October 2004

KOU Zheng, TANG Li-jun, LEI Fu-min, AN Xue-fang, FAN Zhao-jun and LI Tian-xian. Isolation and Identification of an Avian Influenza Virus of Subtype H5N1 from Hubei Province[J]. Virologica Sinica, 2004, 19(5): 490-492.

Citation: KOU Zheng, TANG Li-jun, LEI Fu-min, AN Xue-fang, FAN Zhao-jun, LI Tian-xian. Isolation and Identification of an Avian Influenza Virus of Subtype H5N1 from Hubei Province .VIROLOGICA SINICA, 2004, 19(5) : 490-492.

一株H5N1亚型禽流感病毒的分离与鉴定*

唐利军 ¹ ,
雷富民 ¹ ,
安学芳 ,
范兆军 ¹ ,
李天宪 ¹ ,

1.
1.中国科学院武汉病毒研究所，湖北武汉430071
2.
中国科学院动物研究所，北京 100101

摘要

2004年1月湖北宜昌某鸡场暴发疫病，从该鸡场濒死鸡肺组织中分离到了一株病毒，电镜切片观察到典型的禽流感病毒粒子；采用ELISA检测禽流感抗原为阳性；RT-PCR扩增HA、NA基因并测序，经BLAST分析，HA基因与A/Goose/Guangdong/1/96(H5N1) HA基因同源性为97%；NA基因与A/Goose/Guangdong/1/96(H5N1) NA基因同源性为96％，确定该分离株为禽流感病毒H5N1亚型（A/Chicken/Yichang/Lung-1/04（H5N1））。
- 禽流感病毒
- , H5N1

Isolation and Identification of an Avian Influenza Virus of Subtype H5N1 from Hubei Province

1.
1. Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
2.
Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Corresponding author: LI Tian-xian,

Abstract

A strain of avian influenza virus was isolated from lung tissue in 2004. It was determined to be a Avian influenza virus by ELISA and electron microscopy. HA and NA gene was amplified by RT-PCR and sequenced. By the analyses of HA and NA gene sequences, the isolate was an H5N1 subtype of avian influenza virus (A/Chicken/Yichang/Lung-1/04（H5N1）).
- Avian influenza virus
- , H5N1

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Isolation and Identification of an Avian Influenza Virus of Subtype H5N1 from Hubei Province

Corresponding author: LI Tian-xian,

1. 1. Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
2. Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Abstract: A strain of avian influenza virus was isolated from lung tissue in 2004. It was determined to be a Avian influenza virus by ELISA and electron microscopy. HA and NA gene was amplified by RT-PCR and sequenced. By the analyses of HA and NA gene sequences, the isolate was an H5N1 subtype of avian influenza virus (A/Chicken/Yichang/Lung-1/04（H5N1）).