金黄地鼠是研究动物传染性海绵状脑病的理想模型动物之一,我们利用实时荧光定量RT PCR技术,构建标准重组质粒制备标准曲线,对中枢神经系统的4个不同部位及外周6个组织提取总RNA,反转录后进行PrP基因表达的定量。结果发现,脑的四个检测部位都呈现高的表达量;在外周器官中,淋巴结的表达量和全脑相当,脾脏、心脏、肝脏和肺脏呈现中等程度的表达,肾脏的表达量最低。本研究的结果对于探讨朊蛋白的基本功能和不同组织在传染性海绵状病理发生中的作用,提供了基础数据。

Golden hamster is one of the favored experimental models for studying mechanisms of transmissible spongiform encephalopathies (TSEs). We used the real-time fluorescent RT-PCR technique, constructing the standard plasmid and curve to quantify the PrP gene expression. Total RNA was isolated from four different regions of the brain and six peripheral organs to quantify the gene expression after the reverse transcript. The results showed that high mRNA levels were present in all four regions of the brain examined. In peripheral organs examined, inguinal lymph node showed high level of expression similar to that in brain, spleen, heart, liver and lung showed moderate levels of the expression and kidney showed the lowest expression. Our results provided the basic data of the function of the PrP~c and the effection of different organs in prion diseases.