Isolation and Sequence Analysis of S1 Gene of Avian Infectious Bronchitis Virus A Field  Strain

YANG Jie-hua; DIAO You-xiang; YU Shen-ye; LIU Xia; JIA Shi-jin; LIU Yue-zhu; CHEN Qing-pu

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June 2005

YANG Jie-hua, DIAO You-xiang, YU Shen-ye, LIU Xia, JIA Shi-jin, LIU Yue-zhu and CHEN Qing-pu. Isolation and Sequence Analysis of S1 Gene of Avian Infectious Bronchitis Virus A Field Strain[J]. Virologica Sinica, 2005, 20(3): 283-287.

Citation: YANG Jie-hua, DIAO You-xiang, YU Shen-ye, LIU Xia, JIA Shi-jin, LIU Yue-zhu, CHEN Qing-pu. Isolation and Sequence Analysis of S1 Gene of Avian Infectious Bronchitis Virus A Field Strain .VIROLOGICA SINICA, 2005, 20(3) : 283-287.

鸡传染性支气管炎病毒变异株的分离及其S1基因序列分析

杨杰华 ¹ ,
刁有祥 ^1* ,
于申业 ¹ ,
刘霞 ¹ ,
姜世金 ¹ ,
刘悦竹 ² ,
陈庆普 ²

1.
1.山东农业大学动物科技学院山东泰安 2.山东明发兽药研究所山东济南

摘要

本实验对山东省泰安地区某蛋鸡场产蛋下降鸡群发生的临床症状疑似鸡传染性支气管炎病例,无菌采集气管、肺脏、肾脏等组织,接种SPF鸡胚,经病毒形态观察、血凝特性试验、动物回归试验等证实,我们鉴定到一株鸡传染性支气管炎病毒(IBV);病毒中和试验表明,该病毒为一株变异毒株,使用国外针对IBV793/B血清型发表的血清型特异性引物经RTPCR方法获得了该毒株的免疫原基因S1基因,初步确定该传染性支气管炎病毒变异毒株为793/B血清型。
- 鸡传染性支气管炎病毒
- , TA03分离株
- , S1基因
- , 序列分析

Isolation and Sequence Analysis of S1 Gene of Avian Infectious Bronchitis Virus A Field Strain

Abstract

One strain of Avian Infectious Bronchitis Virus(IBV) was isolated from layer flock which had the symptom of egg drop in Tai’an of Shandong Province, and identified by electron microscope, HA test and animal regressive research. Results of virus neutralization test showed that the isolated virus is a variant strain of IBV. The whole sequence of S1 gene was cloned and analyzed by RT-PCR using specific primers which can differentiate between 793/B IBV and other serotypes of IBV. The strain′s serotype can be primary deduced as 793/B.
- Infectious bronchitis virus（IBV）
- , TA03 strain
- , S1 gene
- , Sequence analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Isolation and Sequence Analysis of S1 Gene of Avian Infectious Bronchitis Virus A Field Strain

Abstract: One strain of Avian Infectious Bronchitis Virus(IBV) was isolated from layer flock which had the symptom of egg drop in Tai’an of Shandong Province, and identified by electron microscope, HA test and animal regressive research. Results of virus neutralization test showed that the isolated virus is a variant strain of IBV. The whole sequence of S1 gene was cloned and analyzed by RT-PCR using specific primers which can differentiate between 793/B IBV and other serotypes of IBV. The strain′s serotype can be primary deduced as 793/B.