Construction and Characterization of SHIV Carrying HIV-1 Capsid

ZHU Yi-xin; LIU Chang; QIAO Wen-tao; CHEN Qi-min; GENG Yun-qi; ZENG Yi

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August 2005

ZHU Yi-xin, LIU Chang, QIAO Wen-tao, CHEN Qi-min, GENG Yun-qi and ZENG Yi. Construction and Characterization of SHIV Carrying HIV-1 Capsid[J]. Virologica Sinica, 2005, 20(4): 346-351.

Citation: ZHU Yi-xin, LIU Chang, QIAO Wen-tao, CHEN Qi-min, GENG Yun-qi, ZENG Yi. Construction and Characterization of SHIV Carrying HIV-1 Capsid .VIROLOGICA SINICA, 2005, 20(4) : 346-351.

替换HIV-1衣壳蛋白基因SHIV的构建及其活性测定

朱义鑫 ^1,2 ,
刘畅 ² ,
乔文涛 ² ,
陈启民 ² ,
耿运琪 ^2* ,
曾毅 ^1,3*

1.
1.中国疾病预防控制中心病毒病预防控制所 2.南开大学生命科学院天津 3.北京北京工业大学

摘要

将猴免疫缺陷病毒(Simianimmunodeficiencyvirus,SIVmm239)中gag基因的衣壳蛋白部分置换成人免疫缺陷病毒(Humanimmunodeficiencyvirustype1,HIV-1HXBc2)的相应部分,构建出替换了衣壳蛋白基因的人/猿嵌合免疫缺陷病毒(SHIV)原病毒DNA。用此SHIV原病毒DNA转染293T细胞,细胞中能够检测到嵌合病毒基因的转录与翻译;在细胞培养液上清中亦可检测到装配出的病毒颗粒。病毒颗粒形态正常,含有基因组RNA,具有反转录酶活性,嵌合的外源衣壳蛋白能够正确剪切,形成棒状的核心。将此嵌合SHIV病毒感染MT4细胞,病毒能够吸附并进入细胞,能完成反转录过程,但不能增殖。
- SHIV
- , SIV
- , HIV-1
- , 衣壳蛋白

Construction and Characterization of SHIV Carrying HIV-1 Capsid

ZHU Yi-xin ^1,2 ,
LIU Chang ² ,
QIAO Wen-tao ² ,
CHEN Qi-min ² ,
GENG Yun-qi ^2* ,
ZENG Yi ^1,3*

Abstract

SHIV (SIV/HIV chimeric virus) proviral DNA carrying Human immunodeficiency (virus) type1 (HIV-1 HXBc2) capsid was constructed on the backbone of Simian immunodeficiency (virus) (SIVmm239). The gene expression of chimeric virus could be detected in 293T cells transfected by SHIV proviral DNA. Chimeric SHIV virions were also obtained in the supernatant of transfected 293T cells. These virions have complete structures,including genomic RNA, reverse transcriptase, core proteins and envelope with glycoproteins. The chimeric Gag precursor could be appropriately cleaved and lead to the conformation of spindle core in the mature virus particles. They could Absorb and enter into MT4 cells and complete the course of reverse transcription. (witbout) repication.
- Simian immunodeficiency virus（SIV）
- , Human immunodeficiency virus（HIV）
- , SIV/HIV chimeric virus（SHIV）
- , Capsid

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Construction and Characterization of SHIV Carrying HIV-1 Capsid

Abstract: SHIV (SIV/HIV chimeric virus) proviral DNA carrying Human immunodeficiency (virus) type1 (HIV-1 HXBc2) capsid was constructed on the backbone of Simian immunodeficiency (virus) (SIVmm239). The gene expression of chimeric virus could be detected in 293T cells transfected by SHIV proviral DNA. Chimeric SHIV virions were also obtained in the supernatant of transfected 293T cells. These virions have complete structures,including genomic RNA, reverse transcriptase, core proteins and envelope with glycoproteins. The chimeric Gag precursor could be appropriately cleaved and lead to the conformation of spindle core in the mature virus particles. They could Absorb and enter into MT4 cells and complete the course of reverse transcription. (witbout) repication.