Comparison of Bioactivity of Two Isolates of Spodoptera exigua Nucleopolyhedrovirus and Application  as Viral Pesticide

ZHANG Hai-yuan; MEI Chun-lei; ZHANG Zhong-xin

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December 2005

ZHANG Hai-yuan, MEI Chun-lei and ZHANG Zhong-xin. Comparison of Bioactivity of Two Isolates of Spodoptera exigua Nucleopolyhedrovirus and Application as Viral Pesticide[J]. Virologica Sinica, 2005, 20(6): 656-659.

Citation: ZHANG Hai-yuan, MEI Chun-lei, ZHANG Zhong-xin. Comparison of Bioactivity of Two Isolates of Spodoptera exigua Nucleopolyhedrovirus and Application as Viral Pesticide .VIROLOGICA SINICA, 2005, 20(6) : 656-659.

SeMNPV两个分离株的生物活性比较及病毒杀虫剂的应用

张海元 ^1,2 ,
梅春蕾 ¹ ,
张忠信 ^1*,,

1.
1.中国科学院武汉病毒研究所湖北武汉 2.长江大学医学院湖北荆州

通讯作者： 张忠信,

摘要

本文对甜菜夜蛾核型多角体病毒的两个分离株(SeMNPV-M,SeMNPV-Z)的生物活性进行了比较,并对甜菜夜蛾核多角体病毒杀虫悬浮剂的田间应用效果进行了评估。病毒生物测定结果显示,SeNPV-M和SeNPV-Z 感染三龄幼虫的半致死剂量(LD50)分别为195．8 PIBs／克饲料和242．4 PIBs／克饲料。使用6000PIB／克饲料的病毒剂量感染三龄幼虫,其半致死时间(LT50)分别为3．50d和3．68d。甜菜夜蛾病毒杀虫悬浮剂已在工厂生产,田间实验结果表明,甜菜夜蛾病毒杀虫悬浮剂可有效控制目标害虫的危害。
- 甜菜夜蛾核型多角体病毒
- , 分离株
- , 病毒杀虫悬浮剂
- , 应用效果

Comparison of Bioactivity of Two Isolates of Spodoptera exigua Nucleopolyhedrovirus and Application as Viral Pesticide

Corresponding author: ZHANG Zhong-xin,

Abstract

The activity of two isolates of Spotoptera exigua nucleopolyhedrovirus , SeMNPV-M and SeMNPV-Z, was compared by bioassays. The LD50 values of SeMNPV-M and SeMNPV-Z in 3rd instar host larvae were 195. 8 PIBs/ gram diet and 242. 4 PIBs/gram diet, respectively. The LT50 values infected with 6000PIBs/gram diet were 3. 50 and 3. 68 days, respectively. The viral suspension formulation pesticide with SeMNPV-Z was produced and in field trials, the SeMNPV pesticide proved to be an effective control agent.
- Spodoptera ezigua nucleopolyhedrovirus
- , SeNPV-M
- , SeNPV-Z
- , viral pesticide

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Comparison of Bioactivity of Two Isolates of Spodoptera exigua Nucleopolyhedrovirus and Application as Viral Pesticide

Corresponding author: ZHANG Zhong-xin,

Abstract: The activity of two isolates of Spotoptera exigua nucleopolyhedrovirus , SeMNPV-M and SeMNPV-Z, was compared by bioassays. The LD50 values of SeMNPV-M and SeMNPV-Z in 3rd instar host larvae were 195. 8 PIBs/ gram diet and 242. 4 PIBs/gram diet, respectively. The LT50 values infected with 6000PIBs/gram diet were 3. 50 and 3. 68 days, respectively. The viral suspension formulation pesticide with SeMNPV-Z was produced and in field trials, the SeMNPV pesticide proved to be an effective control agent.