Mice as a Model for Studying the Avian H5N1 Influenza Virus in Mammals

YANG Song-tao; GAO Yu-wei; WANG Cheng-yu; ZOU Xiao-huan; SU Jian-qing; SUN He-ting; CHANG Shuang; ZHENG Xiao-yi; WANG Tie-cheng; WANG Hua-lei; FENG Na; XIA Xian-zhu

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August 2006

YANG Song-tao, GAO Yu-wei, WANG Cheng-yu, ZOU Xiao-huan, SU Jian-qing, SUN He-ting, CHANG Shuang, ZHENG Xiao-yi, WANG Tie-cheng, WANG Hua-lei, FENG Na and XIA Xian-zhu. Mice as a Model for Studying the Avian H5N1 Influenza Virus in Mammals[J]. Virologica Sinica, 2006, 21(4): 353-357.

Citation: YANG Song-tao, GAO Yu-wei, WANG Cheng-yu, ZOU Xiao-huan, SU Jian-qing, SUN He-ting, CHANG Shuang, ZHENG Xiao-yi, WANG Tie-cheng, WANG Hua-lei, FENG Na, XIA Xian-zhu. Mice as a Model for Studying the Avian H5N1 Influenza Virus in Mammals .VIROLOGICA SINICA, 2006, 21(4) : 353-357.

虎源H5N1亚型禽流感病毒感染小鼠模型的建立

杨松涛 ¹ ,
高玉伟 ¹ ,
王承宇 ¹ ,
邹啸环 ¹ ,
苏建青 ¹ ,
孙贺廷 ¹ ,
常爽 ² ,
郑晓一 ³ ,
王铁成 ¹ ,
王化磊 ¹ ,
冯娜 ¹ ,
夏咸柱 ^1*,,

1.
1.军事医学科学院军事兽医研究所, 吉林长春 130062
2.
吉林大学农学部, 吉林长春 130062
3.
吉林大学生命科学院, 吉林长春 130012

通讯作者： 夏咸柱,

摘要

为研究H5N1亚型禽流感病毒的病原特性、致病机理及对其疫苗与救治药物效果评价提供平台，利用本室分离鉴定的虎源A/Tiger/Harbin/01/2002株（简称HAB/01）H5N1亚型禽流感病毒进行连续10倍稀释后，对4~6周龄雄性BALB/c小鼠经乙醚麻醉后进行滴鼻攻毒，每个稀释度接种10只实验小鼠，测定其MLD50，检测小鼠感染、致病的多项指标，观察期为14d。结果感染小鼠呈现出规律的以肺炎为主的临床症状、病理变化及病死率；测得该病毒对小鼠的MLD50为10-7.1/0.05mL。成功建立了虎源H5N1亚型禽流感病毒感染BALB/c小鼠的实验模型。
- 虎
- , 流感病毒
- , H5N1
- , 小鼠
- , 模型

Mice as a Model for Studying the Avian H5N1 Influenza Virus in Mammals

1.
1.Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China
2.
Division of Agricultural Sciences, Jilin University, Changchun 130062
3.
Life Science Academy of Jilin University, Changchun 130012

Corresponding author: XIA Xian-zhu,

Abstract

The rationale of this study was to elucidate the etiological agent characteristics and pathogenic mechanisms of the Avian H5N1 influenza virus in mammals, and to offer a platform for evaluating vaccines and drugs against AIV. Male BALB/c mice (18~20g) were anesthetized by inhalation of ether and inoculated intranasally with 0.05mL of infectious virus (A/Tiger/Harbin/01/2002) isolating from a tiger. The dose lethal to 50% of mice (MLD50) was determined by infecting groups of ten with serial 10-fold viral dilutions. Mice were observed daily for 14 days for clinical signs of infection and day of survival. The mice showed the typical clinical symptoms, pathological changes and death rates. The MLD50 was about 10-7.1/0.05mL. The data demonstrate that infecting BALB/c mice with the avian H5N1 could serve as a successful model to assess viral infectivity.
- Tiger
- , Influenza virus
- , H5N1
- , BALB/c mice
- , Model

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Mice as a Model for Studying the Avian H5N1 Influenza Virus in Mammals

Corresponding author: XIA Xian-zhu,

1. 1.Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China
2. Division of Agricultural Sciences, Jilin University, Changchun 130062
3. Life Science Academy of Jilin University, Changchun 130012

Abstract: The rationale of this study was to elucidate the etiological agent characteristics and pathogenic mechanisms of the Avian H5N1 influenza virus in mammals, and to offer a platform for evaluating vaccines and drugs against AIV. Male BALB/c mice (18~20g) were anesthetized by inhalation of ether and inoculated intranasally with 0.05mL of infectious virus (A/Tiger/Harbin/01/2002) isolating from a tiger. The dose lethal to 50% of mice (MLD50) was determined by infecting groups of ten with serial 10-fold viral dilutions. Mice were observed daily for 14 days for clinical signs of infection and day of survival. The mice showed the typical clinical symptoms, pathological changes and death rates. The MLD50 was about 10-7.1/0.05mL. The data demonstrate that infecting BALB/c mice with the avian H5N1 could serve as a successful model to assess viral infectivity.