1986 Vol.01(1)

在人们的心目中‘发现’是科学的象徵,新发现往往容易受到重视。因此新闻界以新的发展衡量科学。当诺贝尔提出“诺贝尔奖金”条件时是以新的发现为标准,特别是对人类有益的发现。但是如果认为科学仅是事实的累积就会使人发生误解。不论在生命科学或可能同样地,在其他科学,大部分的主要进展是由于引出了新的概念,或改进了已有的概念。认识世界也可以说是通过概念的改进,而不仅是新事实的发现,虽然两者是有联系的。1953年DNA双螺旋结构的发现使基因有了新的概念。从此遗传学从“经典遗传学”发展为“分子遗传学”。因此在科学进展的过程中必须重视概念的发展。

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THE DEVELOPMENT OF VIRUS CONCEPTS IN 20TH CENTURY

Shang-yin Gao

1986, 1(1): 1

Received: 12 September 2017
Review

EFFECT OF SMALL DOSE INTERFERON OF HUMAN LEUKOCYTES ON THREE VIRAL ANTIGEN OF HEPATITIS B.

XIONG Kai-Jun, Tao-Meng-Lin, WANG Xin-He, JIN Wei-E, FAN Gong-Mei, CENG Lian-Lan, HUANG Hua-Fang, HAN Cai-Yang, Wu Zhang-Qi

1986, 1(1): 8

In this paper.20 cases with hepatitis B including viral carriers ha- ve been treated in a period of three months.The geometric average of HBsAg titer fall from 1:97.01±3.74 to 1:11.5±3.28,P<0.001.13 of them have become negative,accounting for 65 percent of the total,in seven cases with HBeAg positive,six have turned into negative after trea- tment,with the three HBcAg positive Cases,all became negative after a period of treatment,as for control group (polyI:C)there has been no significant difference in p...

APPLICATION OF RADIOIMMUNOPRECIPITATION TO THE TEST OF MONOCLONAL ANTIBODIES OF INFLUENZA VIRUS

LI Gang, ZHANG Peng-Fei, BANG Pei-Xia, WANG Gui-Qiu, LU Bao-Lan

1986, 1(1): 12

This article reports how we applied redioautography of immunopre- cipitation to the test of monoclonal antibodies of influenza virus A/Luo Yang/3/57(H_1N_1).The monoclonal antibodies were prepared from the fusion of the spleen cells of BALB/C mice,immunized with influenza vi- rus,and the myeloma ceils of SPR/O mice.These antibodies were proved specific to hemagglutinin through hemagglutination inhibition test and neuraminidase inhibit on test.The monoclonal antibodies combined pro- tein A-Sepharose then wit...

SCANNING AND TRANSMISSION ELECTRON MICROSCOPIC OBSERVATION ON HSV-2 INFECTED CELLS

CHEN Min-Hui, YAO Xue-Jun, WU Jian-Guo, LIANG Gao-Lin, CHEN Bao-Ping, FANG Ping, LEI Sen-Lin, WANG Qi-Wang, XIAO Tong-Hao

1986, 1(1): 18

Baby rabbit kidney cells infected with 333 strain of HSV-2at an MOI of approximately 3 pfu/cell were fixed at sequential intervals.SEM rev- ealed that large amounts of particles,of which a majority and a mino- rity roughly measured 200 nm(enveloped)and 100 nm(naked)respectiv- ely,appeared freely or in cluster on the whole or on a part of cell sur- face membrane 1 hr post infection(p.i.).The feature of particle dis- tribution remained in the surface of cells 2hrs p.i.and4hrs p.i.However, in the central regio...

OUTBREAK OF ACUTE PHARYNGOCONJUNCTIVAL FEVER CAUSED BY ADENOVIRUS TYPE 7 iN BEIJNIG

HONG Tao, ZHOU He-Mei, XIE Wei-Wei, ZHAO Tong-Xin, ZHOU Jing-Yi, WANG Jie

1986, 1(1): 27

An outbreak of acute pharyngo conjunctival fever occured in a district of Beijing China,in August of 1984.Over 4,000 Pre-school and school children(aged6to13)were affected,and over 80% of the Patients had a history of swiming.Clinically,the illness was characterized by higd fever (39.5-41℃),pharyngitis,conjunctivitis,headache often associated with vomiting.20 samples(10 eye secretions and 10 throat swabs)from 10 patients with typical clinical signs of the illness were collected for virus isolation.14 strain...

IDENTIFICATION AND MOLECULAR CLONING OF DNA OF BUZURA SUPPRESSARIA NUCLEAR POLYHEDROSIS VIRUS

DING Da-Ming, WU Ya-Ming, FANG Ci-Qi, ZHOU Yong-Zhi, SUN Guo-Ping, ZHANG Yi-Min, JIAN Hao-Ran

1986, 1(1): 31

The DNA of Buzura Suppressaria Nuclear Polyhedrosis Virus(BsNPV) was digested at least9 fragments by restriction endonuclease BamHI.The fragments eluted by polyacrylamide gel electrophoresis were recombined with plasmid pBR_(322)incubated with BamHI and transfered into E.coli. Antibiotic screening,electrophoresis analysis and colony hybridization showed the existence of insert sequence in hybrid plasmid.The result suggested that the cloning of BamHI fragments of BsNPV DNA be successful.

STUDY OF PROTEOLYTIC ENZYME ASSOCIATED WITH THE POLYHEDRAL PROTEIN OF Galleria mellonella NUCLEAR POLYHEDROSIS VIRUS

QI Yi-Peng, G.Stainrs

1986, 1(1): 36

Dissolve kinetics for polyhedral matrix protein of Galleria mellonella nuclear polyhedrosis virus was investigated with wide range of pH,diff- erent ionic strength and temperature.A proteolytic enzyme was confir- med with the viral polyhedron.Its optimum pH for dissolved polyhedron is 8.8. The enzymes were purified by precipitaton with(NH_4)_2SO_4 and chrom- atograph with Sephacryl in result a five fold increase of relative enzyme activity.It was lyopholyzed to be a white cotton-form.Three different active ...

A STUDY ON BIOCHEMICAL CHARACTERIZATION OF TAIZISHEN MOSAIC VIRUS

CHEN Li-Hua, CHEN Shen-Liang, SUN Ji-Shan, ZHANG Li-Ren, LIU Qing-Qi

1986, 1(1): 44

The roots tubers of Taizishen are usually used as the traditional Chinese medicine.A rod-shaped virus has been found from the diseased plant of Taizishen. The virus purified by polythylene preciptation and differential cent- rifugation showed rod-shaped,close and numerous particales under the electron microscope,and in one band of existence of these virus particles was further verified by polyacrylamid gel electrophoresis. The molecular weight of coat protein were 16,600 consisting of 161 amino acid residue...

DIAGNOSIS FOR CONGENITAL RUBELLA VIRUS INFECTION BY AN ENZYME-LINKED IMMUNOSORBENT ASSAY

YANG Zhan-Qiu, ZHU Bao-Lian, ZHANG Mei-Ying, XIANG Jin-Min, HUANG Fang-Liao

1986, 1(1): 50

IN VITRO SYNTHESIS OF DNA COMPLEMENTARY TO RNA OF VELVET TOBACCO MOTTLE VIRUS AND SOLANUM NODIFLORUM MOTTLE VIRUS

WU Ya-Ming, YANG Yi-Ping, LIU Yong, DING Da-Ming

1986, 1(1): 53

A PRELIMINARY REVIEW OF TREATING HEPATITIS B BY USING SMALL DOSAGE INTERFERON

YU Hui, WEI Jiu-Xian, XIONG Kai-Jun, WU Zhang-Qi

1986, 1(1): 55