Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells

Yuanzhi Chen; Chenguang Shen; Jing Chen; Junyu Chen; Fentian Chen; Limin Zhang; Xue Liu; Siyuan Chen; Sen Xue; Yongliang Liu; Jixian Tang; Quan Yuan; Yixin Chen; Wenxin Luo; Ningshao Xia

doi:10.1016/j.virs.2022.03.009

August 2022

Yuanzhi Chen, Chenguang Shen, Jing Chen, Junyu Chen, Fentian Chen, Limin Zhang, Xue Liu, Siyuan Chen, Sen Xue, Yongliang Liu, Jixian Tang, Quan Yuan, Yixin Chen, Wenxin Luo and Ningshao Xia. Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells[J]. Virologica Sinica, 2022, 37(4): 619-622. doi: 10.1016/j.virs.2022.03.009

Citation: Yuanzhi Chen, Chenguang Shen, Jing Chen, Junyu Chen, Fentian Chen, Limin Zhang, Xue Liu, Siyuan Chen, Sen Xue, Yongliang Liu, Jixian Tang, Quan Yuan, Yixin Chen, Wenxin Luo, Ningshao Xia. Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells .VIROLOGICA SINICA, 2022, 37(4) : 619-622. http://dx.doi.org/10.1016/j.virs.2022.03.009

在杂交瘤细胞内调控表达活化诱导胞苷脱氨酶（AID）来筛选乙型流感单克隆抗体

陈远志 ^a ,
沈晨光 ^a,b,, ,
陈静 ^a ,
陈俊煜 ^a ,
陈奋天 ^a ,
张丽敏 ^a ,
刘雪 ^a ,
陈思远 ^a ,
薛森 ^a ,
刘永亮 ^a ,
唐济贤 ^a ,
袁权 ^a ,
陈毅歆 ^a,, ,
罗文新 ^a,, ,
夏宁邵 ^a

厦门大学国家传染病诊断试剂与疫苗工程技术研究中心分子疫苗学与分子诊断学国家重点实验室厦门大学公共卫生学院南方医科大学公共卫生学院

通讯作者： 沈晨光, a124965468@smu.edu.cn; 陈毅歆, yxchen2008@xmu.edu.cn; 罗文新, wxluo@xmu.edu.cn;
收稿日期： 2021-09-21

摘要

本研究中，我们构建了一种可以在杂交瘤细胞水平完成的“无克隆操作”抗体类别转换。通过可调控的四环素诱导表达系统（Tet-On）将活化诱导胞苷脱氨酶稳转到一株抗乙型流感的IgM单克隆杂交瘤细胞系，由此我们分离获得7G1全部四种IgG亚型抗体的单克隆细胞系。通过后续的评估实验，我们发现不同类型IgG的7G1抗体体现了不一样的乙型流感广谱结合能力，小鼠动物实验也表明不同类型的7G1抗体针对乙型流感的保护效果也不尽相同。这些抗体的功能差异可能是由于小鼠的Fc不同导致。本研究的方法可用于“按需”对抗体进行细胞水平的类表转换来增强抗体的某些特性，甚至可能存在高频突变。这个改造方法同时可用于抗体药物研发早期，研究抗体在不同亚型状态下的功能。
- 杂交瘤细胞
- , 活化诱导胞苷脱氨酶
- , 乙型流感
- , 单克隆抗体
- , 类别转换

Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells

Yuanzhi Chen ^a ,
Chenguang Shen ^a,b,, ,
Jing Chen ^a ,
Junyu Chen ^a ,
Fentian Chen ^a ,
Limin Zhang ^a ,
Xue Liu ^a ,
Siyuan Chen ^a ,
Sen Xue ^a ,
Yongliang Liu ^a ,
Jixian Tang ^a ,
Quan Yuan ^a ,
Yixin Chen ^a,, ,
Wenxin Luo ^a,, ,
Ningshao Xia ^a

a National Institute of Diagnostics and Vaccine Development in Infectious Diseases, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health & School of Life Science, Xiamen University, Xiamen 361102, China;

Corresponding author: Chenguang Shen, a124965468@smu.edu.cn Yixin Chen, yxchen2008@xmu.edu.cn Wenxin Luo, wxluo@xmu.edu.cn
Received Date: 21 September 2021

Abstract

Highlights
1. Class-switch recombination was mimicked in hybridomas through a controllable expression system of activation-induced cytidine deaminase.
2. IgG antibodies were generated through this system in an anti-Flu B IgM hybridoma 7G1.
3. IgG1 and IgG2a subtypes of 7G1 present improved antiviral activity in vitro and in vivo.

References
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells

Corresponding author: Chenguang Shen, a124965468@smu.edu.cn

Corresponding author: Yixin Chen, yxchen2008@xmu.edu.cn

Corresponding author: Wenxin Luo, wxluo@xmu.edu.cn

a National Institute of Diagnostics and Vaccine Development in Infectious Diseases, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health & School of Life Science, Xiamen University, Xiamen 361102, China;

Received Date: 21 September 2021

Abstract: Highlights
1. Class-switch recombination was mimicked in hybridomas through a controllable expression system of activation-induced cytidine deaminase.
2. IgG antibodies were generated through this system in an anti-Flu B IgM hybridoma 7G1.
3. IgG1 and IgG2a subtypes of 7G1 present improved antiviral activity in vitro and in vivo.

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在杂交瘤细胞内调控表达活化诱导胞苷脱氨酶（AID）来筛选乙型流感单克隆抗体

摘要

Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells

Abstract

References

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Electronic Supplementary Material

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通讯作者: 陈斌, bchen63@163.com