Isolation and oral immunogenicity assessment of porcine epidemic diarrhea virus NH-TA2020 strain: One of the predominant strains circulating in China from 2017 to 2021

Xiaowen Li; Yang Li; Jiapei Huang; Yali Yao; Wenying Zhao; Yunjing Zhang; Jie Qing; Jing Ren; Zhong Yan; Zewei Wang; Xiaofang Hu; Duli Kang; Hongqiang Liu; Zhichun Yan

doi:10.1016/j.virs.2022.08.002

October 2022

. doi: 10.1016/j.virs.2022.08.002

Citation: Xiaowen Li, Yang Li, Jiapei Huang, Yali Yao, Wenying Zhao, Yunjing Zhang, Jie Qing, Jing Ren, Zhong Yan, Zewei Wang, Xiaofang Hu, Duli Kang, Hongqiang Liu, Zhichun Yan. Isolation and oral immunogenicity assessment of porcine epidemic diarrhea virus NH-TA2020 strain: One of the predominant strains circulating in China from 2017 to 2021 .VIROLOGICA SINICA, 2022, 37(5) : 646-655. http://dx.doi.org/10.1016/j.virs.2022.08.002

2017-2021中国猪流行性腹泻主要流行毒株——NH-TA2020株的分离及口服免疫原性评估

李孝文 ^a,c ,
李洋 ^a,c ,
黄佳培 ^a,c ,
姚亚丽 ^b ,
赵文影 ^b ,
张云静 ^b ,
卿杰 ^a,c ,
任景 ^d ,
颜忠 ^a ,
王泽炜 ^a,c ,
胡晓芳 ^a,c ,
康笃利 ^e ,
刘红强 ^e ,
闫之春 ^a,c,,

a 新希望六和股份有限公司, 成都, 610041, 中国;
b 国家兽用药品工程技术研究中心, 洛阳, 471000, 中国;
c 山东新希望六和农牧科技有限公司(新希望六和养猪研究院), 德州, 253034, 中国;
d 猪群健康大数据与智能监测工程实验室, 德州学院, 德州, 253011, 中国;
e 普莱柯生物工程股份有限公司, 洛阳, 471000, 中国

通讯作者： 闫之春, jasonynh@126.com
收稿日期： 2022-03-14
录用日期： 2022-08-02

摘要

猪流行性腹泻病毒（PEDV）引起的猪流行性腹泻（PED）能导致极高的仔猪死亡率，因而PED是全球养猪业最具毁灭性的疾病之一。妊娠母猪通过疫苗免疫，可有效增强肠道-乳腺分泌IgA轴，提高哺乳仔猪获得的乳源免疫水平和抵抗PEDV感染的被动保护水平。本研究为开展PEDV流行病学调查，收集了2017至2021年中国303个猪场的882份腹泻样本。结果分析显示，猪场PEDV阳性检出率为52.15%（158/303），样品PEDV阳性检出率为63.95%（564/882）。对104份PEDV阳性样品的S1基因进行测序，并作系统发育分析，结果显示共有71份（68.27%）样品检出G2c亚型的PEDV，G2c为主要流行亚型。在三个省份发现了一种PEDV新亚型——G2d，其阳性率为9.62%。本研究分离培养了一株G2c亚型毒株——PEDV NH-TA2020，证实其感染仔猪24小时内可引起水样腹泻，表明其致病性较强。妊娠母猪口服NH-TA2020株病毒液后，其初乳中可检测到高水平IgA抗体，其饲喂的仔猪用NH-TA2020株或一株G2d亚型的PEDV毒株——CH-HeB-RY-2020株攻毒后，临床症状和排毒量较对照组明显减少。综上所述，本研究结果表明2017至2021年中国流行的主要PEDV毒株为G2c亚型毒株。口服NH-TA2020株可以增强母猪的IgA和乳源性免疫反应，减少仔猪感染同源或G2d亚型PEDV毒株的风险。
- 猪流行性腹泻病毒
- , 分子流行病学调查
- , 病毒分离
- , 乳源免疫
- , 免疫球蛋白A

Isolation and oral immunogenicity assessment of porcine epidemic diarrhea virus NH-TA2020 strain: One of the predominant strains circulating in China from 2017 to 2021

Xiaowen Li ^a,c ,
Yang Li ^a,c ,
Jiapei Huang ^a,c ,
Yali Yao ^b ,
Wenying Zhao ^b ,
Yunjing Zhang ^b ,
Jie Qing ^a,c ,
Jing Ren ^d ,
Zhong Yan ^a ,
Zewei Wang ^a,c ,
Xiaofang Hu ^a,c ,
Duli Kang ^e ,
Hongqiang Liu ^e ,
Zhichun Yan ^a,c,,

a New Hope Liuhe Co., Ltd., Chengdu, 610041, China;
b National Research Center for Veterinary Medicine, Luoyang, 471000, China;
c Shandong New Hope Liuhe Agriculture and Animal Husbandry Technology Co., Ltd (NHLH Academy of Swine Research), Dezhou, 253034, China;
d Swine Health Data and Intelligent Monitoring Project Laboratory, Dezhou University, Dezhou, 253011, China;
e Pulike Biological Engineering Inc., Luoyang, 471000, China

Corresponding author: Zhichun Yan, jasonynh@126.com
Received Date: 14 March 2022
Accepted Date: 02 August 2022

Abstract

Porcine epidemic diarrhea (PED) caused by porcine epidemic diarrhea virus (PEDV) is one of the most devastating diseases in the global pig industry due to its high mortality rate in piglets. Maternal vaccines can effectively enhance the gut-mammary gland-secretory IgA axis to boost lactogenic immunity and passive protection of nursing piglets against PEDV challenge. From 2017 to 2021, we collected 882 diarrhea samples from 303 farms in China to investigate the epidemiology of PEDV. The result showed that about 52.15% (158/303) of the farms were positive for PEDV with an overall detection rate of 63.95% (564/882) of the samples. The S1 fragments of S gene from 104 strains were sequenced for the phylogenetic analysis. A total of 71 PEDV strains (68.27%) sequenced in this study were clustered into the predominant G2c subgroup, while the newly-defined G2d strains (9.62%) were identified in three provinces of China. The NH-TA2020 strain of G2c subgroup was isolated and cultured, and its infection to piglets caused watery diarrhea within 24 h, indicating its strong pathogenicity. Oral administration of NH-TA2020 strain to pregnant gilts stimulated high levels of IgA antibody in colostrum. The piglets fed by the gilts above were challenged with NH-TA2020 strain or CH–HeB-RY-2020 strain from G2d subgroup, and the clinical symptoms and virus shedding were significantly reduced compared to the mock group. Our findings suggest that G2c subgroup is the predominant branch circulating in China from 2017 to 2021. Oral administration of NH-TA2020 enhances maternal IgA and lactogenic immune responses, which confer protection against the homologous and emerging G2d PEDV strains challenges in neonates.
- Porcine epidemic diarrhea virus (PEDV)
- , Molecular epidemiological investigation
- , Virus isolation
- , Lactogenic immune
- , IgA

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10.1016j.virs.2022.08.002-ESM.docx