Xiaoli Wang, Yu He, Jiaqi Guo, Zhen Wu, Andres Merits, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Xumin Ou, Qun Gao, Di Sun, Anchun Cheng and Shun Chen. Comparative study of the pathogenicity of the mosquito origin strain and duck origin strain of Tembusu virus in ducklings and three-week-old mice[J]. Virologica Sinica, 2023, 38(5): 827-831. doi: 10.1016/j.virs.2023.07.006
Citation: Xiaoli Wang, Yu He, Jiaqi Guo, Zhen Wu, Andres Merits, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Xumin Ou, Qun Gao, Di Sun, Anchun Cheng, Shun Chen. Comparative study of the pathogenicity of the mosquito origin strain and duck origin strain of Tembusu virus in ducklings and three-week-old mice .VIROLOGICA SINICA, 2023, 38(5) : 827-831.  http://dx.doi.org/10.1016/j.virs.2023.07.006

坦布苏病毒蚊源株和鸭源株对雏鸭和3周龄小鼠致病性的比较研究

  • 鸭坦布苏病毒(DTMUV)于2010年出现,属于黄病毒科黄病毒属,是蛋鸭产蛋综合征的病原体。而坦布苏病毒(MM_1775)最先于1955年在马来西亚从三带喙库蚊中分离。本实验将鸭源坦布苏病毒 rCQW1株和蚊源坦布苏病毒rMM_1775株分别感染昆明小鼠和SPF雏鸭。经颅内接种rMM_1775的昆明小鼠表现出典型的神经系统症状,包括厌食、后肢瘫痪、双眼出现黄色粘液、失明和死亡,而经腹腔感染的小鼠未表现出任何症状。经颅内接种和腹腔接种rCQW1的小鼠均未出现任何临床症状。小鼠感染后脑内主要的显微镜病变包括血管扩张和充血,大量神经元变性和坏死。这些结果说明蚊源坦布苏病毒对小鼠的致病性更强。而鸭源坦布苏病毒 rCQW1株对雏鸭的致病性更高,包括更高的死亡率和发病率,并且在心脏、肾脏和肺检测到更高的病毒载量。雏鸭在感染鸭坦布苏病毒rCQW1 3天可检测到病毒血症,而蚊源坦布苏病毒rMM_1775并未检测到,坦布苏病毒为蚊媒黄病毒,这排除了早期蚊源坦布苏病毒在鸭中感染和传播的可能性。鸭感染后显微镜下观察病变多见于脑、脾和胸腺。本研究为了解坦布苏病毒从蚊到鸭的病毒适应度和致病性变化提供了重要参考。

Comparative study of the pathogenicity of the mosquito origin strain and duck origin strain of Tembusu virus in ducklings and three-week-old mice

  • Highlights:1. TMUV can infect Kunming mice by intracranial injection, and the pathogenicity of mosquito TMUV is higher than that of duck TMUV.2. TMUV can infect SPF ducklings by intramuscular injection, and the pathogenicity of duck TMUV is higher than that of mosquito TMUV.3. SPF ducklings infected with mosquito TMUV did not produce viremia at 3, 5 and 7 dpi.

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    1. Chen, S., He, Y., Zhang, R., Liu, P., Yang, C., Wu, Z., Zhang, J., Wang, M., Jia, R., Zhu, D., Liu, M., Yang, Q., Wu, Y., Cheng, A., 2018. Establishment of a reverse genetics system for duck Tembusu virus to study virulence and screen antiviral genes. Antiviral Res 157, 120-127.

    2. Guo, J., He, Y., Wang, X., Jiang, B., Lin, X., Wang, M., Jia, R., Zhu, D., Liu, M., Zhao, X., Yang, Q., Wu, Y., Chen, S., Cheng, A., 2020. Stabilization of a full-length infectious cDNA clone for duck Tembusu virus by insertion of an intron. J Virol Methods 283, 113922.

    3. Kimura, T., Sasaki, M., Okumura, M., Kim, E., Sawa, H., 2010. Flavivirus encephalitis:pathological aspects of mouse and other animal models. Vet Pathol 47, 806-818.

    4. Mao, L., He, Y., Wu, Z., Wang, X., Guo, J., Zhang, S., Wang, M., Jia, R., Zhu, D., Liu, M., Zhao, X., Yang, Q., Mao, S., Wu, Y., Zhang, S., Huang, J., Ou, X., Gao, Q., Sun, D., Cheng, A., Chen, S., 2022. Stem-Loop I of the Tembusu Virus 3'-Untranslated Region Is Responsible for Viral Host-Specific Adaptation and the Pathogenicity of the Virus in Mice. Microbiology spectrum 10, e0244922.

    5. Platt, G.S., Way, H.J., Bowen, E.T., Simpson, D.I., Hill, M.N., Kamath, S., Bendell, P.J., Heathcote, O.H., 1975. Arbovirus infections in Sarawak, October 1968——February 1970 Tembusu and Sindbis virus isolations from mosquitoes. Ann Trop Med Parasitol 69, 65-71.

    6. Prestwood, T.R., May, M.M., Plummer, E.M., Morar, M.M., Yauch, L.E., Shresta, S., 2012. Trafficking and replication patterns reveal splenic macrophages as major targets of dengue virus in mice. J Virol 86, 12138-12147.

    7. Stonedahl, S., Clarke, P., Tyler, K.L., 2020. The Role of Microglia during West Nile Virus Infection of the Central Nervous System. Vaccines (Basel) 8, 485.

    8. Su, J., Li, S., Hu, X., Yu, X., Wang, Y., Liu, P., Lu, X., Zhang, G., Hu, X., Liu, D., Li, X., Su, W., Lu, H., Mok, N.S., Wang, P., Wang, M., Tian, K., Gao, G.F., 2011. Duck egg-drop syndrome caused by BYD virus, a new Tembusu-related flavivirus. PLoS One 6, e18106.

    9. Sun, X., Liu, E., Iqbal, A., Wang, T., Wang, X., Haseeb, A., Ahmed, N., Yang, P., Chen, Q., 2019. The dynamic distribution of duck Tembusu virus in the spleen of infected shelducks. BMC Vet Res 15, 112.

    10. Ti, J., Zhang, M., Li, Z., Li, X., Diao, Y., 2016. Duck Tembusu Virus Exhibits Pathogenicity to Kunming Mice by Intracerebral Inoculation. Front Microbiol 7, 190.

    11. Wang, X., He, Y., Guo, J., Jiang, B., Wang, M., Jia, R., Zhu, D., Liu, M., Zhao, X., Yang, Q., Wu, Y., Zhang, S., Liu, Y., Zhang, L., Yu, Y., Cheng, A., Chen, S., 2021. Construction of an Infectious Clone for Mosquito-Derived Tembusu Virus Prototypical Strain. Virol Sin 36, 1678-1681.

    12. Yan, D., Shi, Y., Wang, H., Li, G., Li, X., Wang, B., Su, X., Wang, J., Teng, Q., Yang, J., Chen, H., Liu, Q., Ma, W., Li, Z., 2018. A Single Mutation at Position 156 in the Envelope Protein of Tembusu Virus Is Responsible for Virus Tissue Tropism and Transmissibility in Ducks. J Virol 92, e00427-18.

    13. Yang, S., Huang, Y., Shi, Y., Bai, X., Yang, P., Chen, Q., 2021. Tembusu Virus entering the central nervous system caused nonsuppurative encephalitis without disrupting the blood-brain barrier. J Virol 95, e02191-20.

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    15. Zhu, K., Huang, J., Jia, R., Zhang, B., Wang, M., Zhu, D., Chen, S., Liu, M., Yin, Z., Cheng, A., 2015. Identification and molecular characterization of a novel duck Tembusu virus isolate from Southwest China. Arch Virol 160, 2781-2790.

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  • 10.1016j.virs.2023.07.006-ESM.docx

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    Comparative study of the pathogenicity of the mosquito origin strain and duck origin strain of Tembusu virus in ducklings and three-week-old mice

      Corresponding author: Anchun Cheng, chenganchun@vip.163.com
      Corresponding author: Shun Chen, shunchen@sicau.edu.cn
    • a. Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China;
    • b. Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China;
    • c. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China;
    • d. Institute of Technology, University of Tartu, Tartu, 999148, Estonia

    Abstract: Highlights:1. TMUV can infect Kunming mice by intracranial injection, and the pathogenicity of mosquito TMUV is higher than that of duck TMUV.2. TMUV can infect SPF ducklings by intramuscular injection, and the pathogenicity of duck TMUV is higher than that of mosquito TMUV.3. SPF ducklings infected with mosquito TMUV did not produce viremia at 3, 5 and 7 dpi.

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