ESTABLISHMENT OF HYBRIDOMA CELL LINES SECRETING MCAB TO JEV AND THEIR USES FOR ANTIGENIC ANALYSIS

SHU Yong-Xin; LI De-Fu; LI Xiu-Hua; WANG Jin-Feng; ZHANG Guo-Ming; GUO Wei

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June 1986

SHU Yong-Xin, LI De-Fu, LI Xiu-Hua, WANG Jin-Feng, ZHANG Guo-Ming and GUO Wei. ESTABLISHMENT OF HYBRIDOMA CELL LINES SECRETING MCAB TO JEV AND THEIR USES FOR ANTIGENIC ANALYSIS[J]. Virologica Sinica, 1986, 1(3).

Citation: SHU Yong-Xin, LI De-Fu, LI Xiu-Hua, WANG Jin-Feng, ZHANG Guo-Ming, GUO Wei. ESTABLISHMENT OF HYBRIDOMA CELL LINES SECRETING MCAB TO JEV AND THEIR USES FOR ANTIGENIC ANALYSIS .VIROLOGICA SINICA, 1986, 1(3) : 19.

乙型脑炎病毒单克隆杂交瘤抗体细胞系的建立及其应用于抗原性比较

1.
卫生部药品生物制品检定所
2.
卫生部药品生物制品检定所北京

摘要

本文建立了13侏乙脑单克隆抗体杂交瘤细胞系,对其中6株进行了特性研究,结果表明该6株中3株为乙脑病毒种特异性抗体,3株为乙脑病毒亚组特异性抗体。以免疫荧光技术对12株国内外分离的乙脑病毒进行了抗原性比较,结果表明我国分离的大多数病毒株间无明显的抗原性差异,但个别近年来分离的病毒侏如云南的Y-M60,福建的NY,湖南的X8103株与1949年北京分离的P_3株即目前用于灭活疫苗生产的病毒株间有一定的差异。我国的大多数病毒株与日本的中山(NaK)株则有明显的差别。此外,并发现2株单克隆抗体对我国的全部野病毒株有高滴度的强荧光反应而对14-2减毒株仅有十分微弱反应,甚至无反应。以上结果提示乙脑不同病毒株间存在抗原性差异,但是否有免疫保护上的抗原性差异尚待进一步研究。

ESTABLISHMENT OF HYBRIDOMA CELL LINES SECRETING MCAB TO JEV AND THEIR USES FOR ANTIGENIC ANALYSIS

1.
National Institute for the Control of Pharmaceutical and Biological Products
2.
Beijing

Abstract

Thriteen hybridoma cell lines secreting McAbs against IEV have been established. Six of them were selected for study. The characterization of the six cell lines of McAb indicated that three of them were JEV—species specific and the other three were JEV—subgroup specific. In this paper, 12 JE virus strains, including 10 Chinese strains, one Japanese strain and one attenuated virus, were selected for antigenic analysis with the six cell lines of McAb. The result showed that most of those Chinese strains were ...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

ESTABLISHMENT OF HYBRIDOMA CELL LINES SECRETING MCAB TO JEV AND THEIR USES FOR ANTIGENIC ANALYSIS

1. National Institute for the Control of Pharmaceutical and Biological Products
2. Beijing

Abstract: Thriteen hybridoma cell lines secreting McAbs against IEV have been established. Six of them were selected for study. The characterization of the six cell lines of McAb indicated that three of them were JEV—species specific and the other three were JEV—subgroup specific. In this paper, 12 JE virus strains, including 10 Chinese strains, one Japanese strain and one attenuated virus, were selected for antigenic analysis with the six cell lines of McAb. The result showed that most of those Chinese strains were ...