THE AVAILABILITY OF MONOCLONAL ANTIBODY IN DETECTING HERPES SIMPLEX VIRUS ANTIGEN WITH ELISA

SHEN Qian; TANG Jia-Qi; QIN Ke-Feng; HONG Mei-Xian; JIANG Chao-Zhun

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June 1986

SHEN Qian, TANG Jia-Qi, QIN Ke-Feng, HONG Mei-Xian and JIANG Chao-Zhun. THE AVAILABILITY OF MONOCLONAL ANTIBODY IN DETECTING HERPES SIMPLEX VIRUS ANTIGEN WITH ELISA[J]. Virologica Sinica, 1986, 1(3).

Citation: SHEN Qian, TANG Jia-Qi, QIN Ke-Feng, HONG Mei-Xian, JIANG Chao-Zhun. THE AVAILABILITY OF MONOCLONAL ANTIBODY IN DETECTING HERPES SIMPLEX VIRUS ANTIGEN WITH ELISA .VIROLOGICA SINICA, 1986, 1(3) : 31.

单克隆抗体在酶联免疫吸附实验检测单纯疱疹病毒抗原中的实用性

1.
第四军医大学微生物学教研室
2.
第四军医大学微生物学教研室西安

摘要

本文采用ELISA双夹心法,通过对70份临床送检标本HSV抗原的检测,评价了单克隆抗体(McAb)在快速检测HSV抗原中的实用性。McAb ELISA法的敏感性为20～30TCID50/0.1ml,临床送检标本病毒抗原的检出与病毒分离结果的一致率为85％。两种McAb混合使用,可以提高方法的敏感性。

THE AVAILABILITY OF MONOCLONAL ANTIBODY IN DETECTING HERPES SIMPLEX VIRUS ANTIGEN WITH ELISA

1.
Department of Microbiology
2.
The Fourth Military Medical College

Abstract

A double sandwich ELISA has been developed to detect herpes simplex virus(HSV) antigen in 70 clinical specimens, A valuation on availability of Monoclonal Anti body (McAb) in rapidly detecting HSV antigen was set, The sensitivity of McAb ELISA was 20-30 TCID50/0.1ml. The concordance of detecting HSV between McAb ELISA and virus isolation in clinical specimens was 85％. The mixture of two McAbs was used, it might elevate the sensitivity of ELISA.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

THE AVAILABILITY OF MONOCLONAL ANTIBODY IN DETECTING HERPES SIMPLEX VIRUS ANTIGEN WITH ELISA

1. Department of Microbiology
2. The Fourth Military Medical College

Abstract: A double sandwich ELISA has been developed to detect herpes simplex virus(HSV) antigen in 70 clinical specimens, A valuation on availability of Monoclonal Anti body (McAb) in rapidly detecting HSV antigen was set, The sensitivity of McAb ELISA was 20-30 TCID50/0.1ml. The concordance of detecting HSV between McAb ELISA and virus isolation in clinical specimens was 85％. The mixture of two McAbs was used, it might elevate the sensitivity of ELISA.