DETECTION OF HEPATITIS A ANTIGEN BY A CELL CULTURE FLUORESCENT ANTIBODY TECHNIQUE WITH BIOTIN-AVIDIN

ZHANG Hua-Yuan; RICHARD J.DAEMER

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June 1987

ZHANG Hua-Yuan and RICHARD J.DAEMER. DETECTION OF HEPATITIS A ANTIGEN BY A CELL CULTURE FLUORESCENT ANTIBODY TECHNIQUE WITH BIOTIN-AVIDIN[J]. Virologica Sinica, 1987, 2(3).

Citation: ZHANG Hua-Yuan, RICHARD J.DAEMER. DETECTION OF HEPATITIS A ANTIGEN BY A CELL CULTURE FLUORESCENT ANTIBODY TECHNIQUE WITH BIOTIN-AVIDIN .VIROLOGICA SINICA, 1987, 2(3) : 13.

抗生物素蛋白-生物素免疫荧光技术检测细胞培养中增殖的甲型肝炎病毒抗原

1.
卫生部药品生物制品检定所
2.
Center for Drugs and Biologics FDA U. S. A.

摘要

检测细胞培养增殖甲型肝炎病毒抗原(HAAg)的敏感方法主要有直接免疫荧光(DIF),间接免疫荧光(IDIF),固相放射免疫(RIA)及放射免疫斑分析(RIFA)等。本文第一次报告用抗生物素蛋白-生物素(Avidin-Biotin)放大作用提高免疫荧光技术的敏感性应用在细胞培养中检测HAAg,生物素化抗甲肝IgG与抗生物素蛋白联结的荧光素偶合检测(BA-FA)与DIF比较,BA-FA不仅可获得背景清晰的荧光。而且快速、节省特异抗体,BA-FA特异性亦好。

DETECTION OF HEPATITIS A ANTIGEN BY A CELL CULTURE FLUORESCENT ANTIBODY TECHNIQUE WITH BIOTIN-AVIDIN

1.
National Institute for the Control of Pharmaceutical and Biological Products
2.
BeijingNational Center for Drugs and Biologics

Abstract

This paper describes an improved fluorescent antibody technique for the detection of HAV. The assay is unique in its use of the characteristic of a highaffinity complex between fluorescence-conjugated avidin and biotin-linked anti-HAV to increase sensitivity. We have made a comparison between direct-fluorescent and fluorescentantibody technique with biotin-avidin with same lot IgG antibody and same lot ACMK inoculated HAV. Because of the high binding affinity of this system due to multiple binding of biotin...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

DETECTION OF HEPATITIS A ANTIGEN BY A CELL CULTURE FLUORESCENT ANTIBODY TECHNIQUE WITH BIOTIN-AVIDIN

1. National Institute for the Control of Pharmaceutical and Biological Products
2. BeijingNational Center for Drugs and Biologics

Abstract: This paper describes an improved fluorescent antibody technique for the detection of HAV. The assay is unique in its use of the characteristic of a highaffinity complex between fluorescence-conjugated avidin and biotin-linked anti-HAV to increase sensitivity. We have made a comparison between direct-fluorescent and fluorescentantibody technique with biotin-avidin with same lot IgG antibody and same lot ACMK inoculated HAV. Because of the high binding affinity of this system due to multiple binding of biotin...