ISOLATION AND SOME PROPERTIES OF AN ALKALINE PROTEASE IN NUCLEAR POLYHEDROSIS VIRUS OF ATTACUS RICINI

LIN Qi-Feng; LU Wen-Jun

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June 1987

LIN Qi-Feng and LU Wen-Jun. ISOLATION AND SOME PROPERTIES OF AN ALKALINE PROTEASE IN NUCLEAR POLYHEDROSIS VIRUS OF ATTACUS RICINI[J]. Virologica Sinica, 1987, 2(3).

Citation: LIN Qi-Feng, LU Wen-Jun. ISOLATION AND SOME PROPERTIES OF AN ALKALINE PROTEASE IN NUCLEAR POLYHEDROSIS VIRUS OF ATTACUS RICINI .VIROLOGICA SINICA, 1987, 2(3) : 55.

蓖麻蚕核型多角体碱性蛋白酶的分离与性质

林栖凤 ,
卢文筠

1.
武汉大学病毒研究所
2.
武汉大学病毒研究所武汉

摘要

本文证明在蓖麻蚕核多角体中也存在着碱性蛋白酶,该酶在Na_2CO_3碱性溶液中能使多角体蛋白质降解,最适pH为1112,最适温度约为40℃,在60℃以上严重失活,重金属Hg~(+2),Cu~(+2)离子对酶活性有较强的抑制作用,而Na~+,Mg~(+2)离子影响不大。本文采用ConA-Sepharose亲和层折一步分离了蓖麻蚕核多角体碱性蛋白酶,简化了分离过程。

ISOLATION AND SOME PROPERTIES OF AN ALKALINE PROTEASE IN NUCLEAR POLYHEDROSIS VIRUS OF ATTACUS RICINI

LIN Qi-Feng ,
LU Wen-Jun

1.
Department of Virology
2.
Wuhan University

Abstract

The paper confirmed the presence of alkaline protease in the nuclear polyhedra of the Attacus ricini and the characteristics of the protease. When the nuclear polyhedra was exposed to alkaline solution, a proteolytic enzyme in the polyhedra degraded the major structural proteins of polyhedra into proteins with the lower molecular weight, The proteolytic activity showed an optimum temperature of 40℃ and an optimum pH of 11 to 12. The activity was inhibited by metallic ions such as Hg~(2+) and Cu~(2+). The en...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

ISOLATION AND SOME PROPERTIES OF AN ALKALINE PROTEASE IN NUCLEAR POLYHEDROSIS VIRUS OF ATTACUS RICINI

LIN Qi-Feng ,
LU Wen-Jun

1. Department of Virology
2. Wuhan University

Abstract: The paper confirmed the presence of alkaline protease in the nuclear polyhedra of the Attacus ricini and the characteristics of the protease. When the nuclear polyhedra was exposed to alkaline solution, a proteolytic enzyme in the polyhedra degraded the major structural proteins of polyhedra into proteins with the lower molecular weight, The proteolytic activity showed an optimum temperature of 40℃ and an optimum pH of 11 to 12. The activity was inhibited by metallic ions such as Hg~(2+) and Cu~(2+). The en...