ELIMINATION OF CITRUS INFECTIOUS VARIEGATION VIRUS(CVV) BY SHOOT-TIP GRAFTING IN VITRO

GUO Zhi-Yong; A.Starrantino; M.Davino

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June 1987

GUO Zhi-Yong, A.Starrantino and M.Davino. ELIMINATION OF CITRUS INFECTIOUS VARIEGATION VIRUS(CVV) BY SHOOT-TIP GRAFTING IN VITRO[J]. Virologica Sinica, 1987, 2(3).

Citation: GUO Zhi-Yong, A.Starrantino, M.Davino. ELIMINATION OF CITRUS INFECTIOUS VARIEGATION VIRUS(CVV) BY SHOOT-TIP GRAFTING IN VITRO .VIROLOGICA SINICA, 1987, 2(3) : 72.

柑桔传染性杂色花叶病(CVV)的茎尖嫁接脱毒

1.
广东农业科学院果树研究所
2.
意大利柑桔研究所
3.
意大利Catania大学植物病理研究所广州

摘要

通过茎尖嫁接,使4个被传染性杂色花叶病(CVV)侵染的柑桔品种脱毒。试验结果表明,ELISA(enzyme-linked immunosorbent assay)检测比指示植物接种诊断更为灵敏、快速。茎尖嫁接前,利用6-苄氨基嘌吟(BAP)或2.4-D处理茎尖和砧木切口,嫁接成活率可达86％—90％。

ELIMINATION OF CITRUS INFECTIOUS VARIEGATION VIRUS(CVV) BY SHOOT-TIP GRAFTING IN VITRO

1.
The Fruit Tree Research Institute of Guangdong Academy of Agricultural Sciences
2.
GuangzhouExperiment Institute of Citrus Culture
3.
Italy Institute of Plant Pathology

Abstract

Four varieties of citrus infected with Citrus Infectious Variegation Virus(CVV) were freed from the virus by shoot-tip grafting in vitro. The tests carried out by enzyme-linked immunosorbent assay(ELISA) were faster and more sensitive than those made by graft-inoculation to indicator plants. The survival rate of grafting was up to 85-90% if shoot-tip and cut of rootstock were treated with 6-benylaminopurine(BAP) or 2, 4-D before grafting.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

ELIMINATION OF CITRUS INFECTIOUS VARIEGATION VIRUS(CVV) BY SHOOT-TIP GRAFTING IN VITRO

1. The Fruit Tree Research Institute of Guangdong Academy of Agricultural Sciences
2. GuangzhouExperiment Institute of Citrus Culture
3. Italy Institute of Plant Pathology

Abstract: Four varieties of citrus infected with Citrus Infectious Variegation Virus(CVV) were freed from the virus by shoot-tip grafting in vitro. The tests carried out by enzyme-linked immunosorbent assay(ELISA) were faster and more sensitive than those made by graft-inoculation to indicator plants. The survival rate of grafting was up to 85-90% if shoot-tip and cut of rootstock were treated with 6-benylaminopurine(BAP) or 2, 4-D before grafting.