STUDIES ON METHOD FOR QUANTITATIVE DETECTION OF THE POPULATION OF PSEUDOMONAS SOLANACEARUM BY PHAGE TECHNIQUE Ⅰ. Quantitatve Detecting Method of Pseudomonas solanacearum Bacteria by Phage Zp-2

LIN Xin-Zheng; MO Yin-Yuan; FANG Zhong-Da

PDF
Cite
Share

facebook

twitter

google

linkedin

June 1987

LIN Xin-Zheng, MO Yin-Yuan and FANG Zhong-Da. STUDIES ON METHOD FOR QUANTITATIVE DETECTION OF THE POPULATION OF PSEUDOMONAS SOLANACEARUM BY PHAGE TECHNIQUE Ⅰ. Quantitatve Detecting Method of Pseudomonas solanacearum Bacteria by Phage Zp-2[J]. Virologica Sinica, 1987, 2(3).

Citation: LIN Xin-Zheng, MO Yin-Yuan, FANG Zhong-Da. STUDIES ON METHOD FOR QUANTITATIVE DETECTION OF THE POPULATION OF PSEUDOMONAS SOLANACEARUM BY PHAGE TECHNIQUE Ⅰ. Quantitatve Detecting Method of Pseudomonas solanacearum Bacteria by Phage Zp-2 .VIROLOGICA SINICA, 1987, 2(3) : 86.

噬菌体定量检测青枯段单胞杆菌(Pseudomonas solanacearum)方法的研究 Ⅰ、噬菌体zp—2对寄主细菌定量测定的研究

1.
南京农业大学植病组
2.
南京农业大学植病组南京

摘要

本文报道利用噬菌体zp-2对青枯假单胞杆菌姜瘟菌株定量检测方法的研究。通过对纯菌的测定,结果表明在每毫升10~3菌落形成单位(CFU)的浓度下,纯菌回收率达81％,检测的敏感性较高,在每毫升10~2(CFU)的条件下也能测到活菌。

STUDIES ON METHOD FOR QUANTITATIVE DETECTION OF THE POPULATION OF PSEUDOMONAS SOLANACEARUM BY PHAGE TECHNIQUE Ⅰ. Quantitatve Detecting Method of Pseudomonas solanacearum Bacteria by Phage Zp-2

1.
Nanjing Agricultural Univ. Dept. of Plant Protection
2.
Nanjing

Abstract

The present paper deals with the phage technique to determine the Pseudomonas solanacearum bacteria quantitatively, Results show that mean recovery of pure bacteria cells was 81% at concentration of 10~3 colony formation unit(CFU)per ml of phage Zp-2 isolate. The optimun adsorption temperature of Zp-2 is 30℃. and optimun adsorption time is 30min. It is a high sensitive method for detecting Pseudomonas solanacearum.

References
Proportional views

PDF

Article Metrics

Article views(3467) PDF downloads(716) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

STUDIES ON METHOD FOR QUANTITATIVE DETECTION OF THE POPULATION OF PSEUDOMONAS SOLANACEARUM BY PHAGE TECHNIQUE Ⅰ. Quantitatve Detecting Method of Pseudomonas solanacearum Bacteria by Phage Zp-2

1. Nanjing Agricultural Univ. Dept. of Plant Protection
2. Nanjing

Abstract: The present paper deals with the phage technique to determine the Pseudomonas solanacearum bacteria quantitatively, Results show that mean recovery of pure bacteria cells was 81% at concentration of 10~3 colony formation unit(CFU)per ml of phage Zp-2 isolate. The optimun adsorption temperature of Zp-2 is 30℃. and optimun adsorption time is 30min. It is a high sensitive method for detecting Pseudomonas solanacearum.