The Study of Some Physico-Chemical Properties of the Polyhedrin of Heliothis armigera Nuclear Polyhedrosis Virus VHA-273 Strain

WU Bai-Chun

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June 1990

WU Bai-Chun. The Study of Some Physico-Chemical Properties of the Polyhedrin of Heliothis armigera Nuclear Polyhedrosis Virus VHA-273 Strain[J]. Virologica Sinica, 1990, 5(3).

Citation: WU Bai-Chun. The Study of Some Physico-Chemical Properties of the Polyhedrin of Heliothis armigera Nuclear Polyhedrosis Virus VHA-273 Strain .VIROLOGICA SINICA, 1990, 5(3) : 294.

中国棉铃虫核型多角体病毒VHA 273毒株多角体蛋白质某些理化特性的研究

吴柏春

1.
华中师范大学生物系武汉

摘要

从VHA273病毒感染中国棉铃虫的典型病虫尸抽提纯化多角体。经碱解释放的多角体蛋白,用超速离心,Sepharose 6B柱层析纯化,多种方法鉴定其纯度。SDS-PAGE测出多角体蛋白亚基分子量MW=28,000±500d。等电聚焦电泳测出7条电泳带,其主带p15.78±0.02,次带p15.3S-6.25。氨基酸组成分析表明富含酸性氨基酸。Niles兰染脂蛋白、甲苯胺兰染糖蛋白结果均呈阳性。
- HaNPV
- , VHA273
- , 多角体蛋白

The Study of Some Physico-Chemical Properties of the Polyhedrin of Heliothis armigera Nuclear Polyhedrosis Virus VHA-273 Strain

WU Bai-Chun

1.
Hua Zong Normal University

Abstract

Polyhedra were extracted from the cadavers of Heliothis armigera infected with VHA-273 strain of Heliothis armigera nuclear polyhedrosis virus. Observation under electron microscope showed that the polyhedra consisted of mNPV (90%) and sNPV(10%). The purity of polyhedrin purified by serial procedures was confirmed by immunological and electrophoresis methods. When the isoelectric point of the polyhedrin was determined by isoelectric focusing electrophoresis, 7bands appeared, with a major band at p15.78±0.02...
- Heliothis armigera nuclear polyhedrosis virus VHA-273 Polyhedrin

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Study of Some Physico-Chemical Properties of the Polyhedrin of Heliothis armigera Nuclear Polyhedrosis Virus VHA-273 Strain

WU Bai-Chun

1. Hua Zong Normal University

Abstract: Polyhedra were extracted from the cadavers of Heliothis armigera infected with VHA-273 strain of Heliothis armigera nuclear polyhedrosis virus. Observation under electron microscope showed that the polyhedra consisted of mNPV (90%) and sNPV(10%). The purity of polyhedrin purified by serial procedures was confirmed by immunological and electrophoresis methods. When the isoelectric point of the polyhedrin was determined by isoelectric focusing electrophoresis, 7bands appeared, with a major band at p15.78±0.02...