The Purification of Citrus Tristeza Virus

LI Kai-Ben; YANG Su; WU Ru-Jian; XU Jie; KE Chong

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June 1990

LI Kai-Ben, YANG Su, WU Ru-Jian, XU Jie and KE Chong. The Purification of Citrus Tristeza Virus[J]. Virologica Sinica, 1990, 5(3).

Citation: LI Kai-Ben, YANG Su, WU Ru-Jian, XU Jie, KE Chong. The Purification of Citrus Tristeza Virus .VIROLOGICA SINICA, 1990, 5(3) : 312.

柑桔衰退病毒的提纯

1.
福建省农科院中心实验室
2.
福建省农科院中心实验室福州

摘要

从甜橙病株的嫩梢皮组织提纯柑桔衰退病毒(Citrus Tristeza Virus,CTV),冰冻组织按每克鲜组织加入5ml0.1mol/L Tris缓冲液pH8.4(内含0.15％Triton x—100)进行匀浆。经几次差速离心和两次PEG(分子量6,000)沉淀后,将获得的病毒粗提液铺在不连续蔗糖密度梯度液上,HITACHI RPS_(40)T转头30,000r/m离心3小时,收集位于300mg/ml和400mg/ml梯度层之间的分离带,洗脱、浓缩后获得CTV提纯物。提纯的CTV粒子大小为1.000—1,500x12urn,与美国的CTV抗血清起阳性反应。
- 柑桔
- , 衰退病
- , 提纯

The Purification of Citrus Tristeza Virus

1.
Fujian Academy of Agricultural Sciences
2.
Fushou 350003

Abstract

This paper deals with the Purification method of citrus tristeza virus (CTV) from infected sweet orange(Citrus sinensis[L]. Osbeck). The frozen and infected tissues were homogenized with 0.1mol/L Tris-buffer pH8.4 which contains 0.15% TritonX-100. After several differential centrifugations including two times of precipitation with PEG (MW 6,000), the supernatant was centrifuged by incontinuous sucrose density gradient of 200—400mg/ml and 600 mg/ml at 30,000 r/m (Hitachi, RPT 40 rot) for 3 hours. Then the ba...
- Citrus Tristeza Virus Purification

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Purification of Citrus Tristeza Virus

1. Fujian Academy of Agricultural Sciences
2. Fushou 350003

Abstract: This paper deals with the Purification method of citrus tristeza virus (CTV) from infected sweet orange(Citrus sinensis[L]. Osbeck). The frozen and infected tissues were homogenized with 0.1mol/L Tris-buffer pH8.4 which contains 0.15% TritonX-100. After several differential centrifugations including two times of precipitation with PEG (MW 6,000), the supernatant was centrifuged by incontinuous sucrose density gradient of 200—400mg/ml and 600 mg/ml at 30,000 r/m (Hitachi, RPT 40 rot) for 3 hours. Then the ba...