JI Ming-Chun, ZHOU Yao-Xi and YAO Kun. Specific HSV-I Antibody Production in Vitro by Human Peripheral Lymphocytes andEnhancement by IFNγ[J]. Virologica Sinica, 1991, 6(3).
Citation:
JI Ming-Chun, ZHOU Yao-Xi, YAO Kun.
Specific HSV-I Antibody Production in Vitro by Human Peripheral Lymphocytes andEnhancement by IFNγ .VIROLOGICA SINICA, 1991, 6(3)
: 207.
Ⅰ型单纯疱疹病毒(HSV—Ⅰ)体外抗体应答及IFNr的调节
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1.
扬州医学院微生物学教研室
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2.
南京医学院微生物学教研室
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南京医学院微生物学教研室 扬州
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摘要
以纯化、灭活的HSV-I为抗原,体外致敏洗涤过的健康成人外周血淋巴细胞,37℃孵育12天,细胞培养上清中的特异性抗体用ELISA法测定。11例血清HSV-I抗体阳性成人的淋巴细胞接受灭活HSV-I抗原致敏后,培养上清中均能检出特异性抗体,抗体类型为IgG(26.6土23ng/m1),体外产生的特异抗体水平与原血清抗体水平无相关性(R=0.45,P>0.05)。同法刺激新生儿淋巴细胞;不能诱生任何类型的HSV-I抗体。在本实验系统中,特异性抗体应答是一个蛋白质的全新(de novo)合成过程,应答水平和体外致敏用的病毒抗原量有明显剂量依赖关系,最适刺激抗原量为108ng/ml。HSV-I抗体应答需要T、B细胞的相互作用,两类细胞单独均不能诱导特异性HSV-I抗体应答。在本实验系统中加入适量重组人γ干扰素,能增强抗体应答水平,过高剂量起抑制作用。
Specific HSV-I Antibody Production in Vitro by Human Peripheral Lymphocytes andEnhancement by IFNγ
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Abstract
Human peripheral blood lymphocytes from normal adult donors were stimulated in vitro by UV-inactived Herpes simplex virus type Ⅰ(HSV-Ⅰ) as an antingen, incubated in 37℃ for 12 days. Specific anti-HSV-Ⅰ antibody was measured by ELISA Anti-HSV antibody of IgG type was produced in vitro in all 11 individuals (26.6±23ng/ml) whose sera were sero-positive to HSV-Ⅰ. Newborn lymphocyte cultures failed to induce anti-HSV-Ⅰantibody of IgG or IgM type after stimulation. The anti-HSV-I antibody response of lympb0cytes ...
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