The cDNA Cloning and Nucleotide Sequence of the Minor Capsid Gene of Rice Dwarf Virus

YE Yin; ZHAO Feng; GAO Ju; TIAN Bo

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August 1991

YE Yin, ZHAO Feng, GAO Ju and TIAN Bo. The cDNA Cloning and Nucleotide Sequence of the Minor Capsid Gene of Rice Dwarf Virus[J]. Virologica Sinica, 1991, 6(4).

Citation: YE Yin, ZHAO Feng, GAO Ju, TIAN Bo. The cDNA Cloning and Nucleotide Sequence of the Minor Capsid Gene of Rice Dwarf Virus .VIROLOGICA SINICA, 1991, 6(4) : 381.

水稻矮缩病毒小外壳蛋白基因的合成、克隆和序列分析

1.
中国科学院微生物研究所
2.
中国医学科学院药物研究所
3.
中国科学院微生物研究所北京

摘要

从感染水稻矮缩病毒(RDV)的水稻叶片中分离纯化了RDV,用人工合成的寡核苷酸为引物,合成了长度为1.0kb的小外壳蛋白基因,经PCR扩增后,克隆至pUC-19载体上。以双脱氧链终止法测序得到其全长序列,同已发表的RDV日本分离物小外壳蛋白基因序列相比有很高的同源率。
- 水稻矮缩病毒
- , 小外壳蛋白基因
- , PCR
- , 序列

The cDNA Cloning and Nucleotide Sequence of the Minor Capsid Gene of Rice Dwarf Virus

1.
Beijing Institute of Microbiology
2.
Academia Sinica
3.
Beijing 100080

Abstract

Rice Dwarf Virus was isolated and purified from infected rice leaves. The cDNA of the minor capsid gene was synthesized with synthetic oligonucleotides as primers and amplified by PCR The cDNA sequence of the minor capsid gene was determined and has high homology to that of Japanese isolate.
- Rice Dwarf Virus
- , Minor capsid gene
- , PCR
- , Sequence

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The cDNA Cloning and Nucleotide Sequence of the Minor Capsid Gene of Rice Dwarf Virus

1. Beijing Institute of Microbiology
2. Academia Sinica
3. Beijing 100080

Abstract: Rice Dwarf Virus was isolated and purified from infected rice leaves. The cDNA of the minor capsid gene was synthesized with synthetic oligonucleotides as primers and amplified by PCR The cDNA sequence of the minor capsid gene was determined and has high homology to that of Japanese isolate.