Preparation and Application of Biotin-labelled Human Cytomegalovirus Nucleic Acid Probe

CHENG Li; CHEN Man; CHEN Wen-Kai; GUO Shu-Fang

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February 1993

CHENG Li, CHEN Man, CHEN Wen-Kai and GUO Shu-Fang. Preparation and Application of Biotin-labelled Human Cytomegalovirus Nucleic Acid Probe[J]. Virologica Sinica, 1993, 8(1).

Citation: CHENG Li, CHEN Man, CHEN Wen-Kai, GUO Shu-Fang. Preparation and Application of Biotin-labelled Human Cytomegalovirus Nucleic Acid Probe .VIROLOGICA SINICA, 1993, 8(1) : 25.

人巨细胞病毒的生物素DNA探针的制备和应用

1.
湖北医学院病毒研究所
2.
湖北医学院病毒研究所武汉

摘要

本研究用克隆的HCMV AD169株DNA片段,制备了生物素标记的DNA探针,建立了检测临床脐带血、尿标本中HCMV DNA的核酸探针杂交方法。该探针可测出100pg同源DNA,不与人胚肺细胞、Hep-2细胞DNA以及其他疱疹病毒的DNA发生反应。用核酸杂交方法检测了30份脐带血标本,有11例阳性,阳性率为33%。10例孕妇尿标本中,3例阳性,阳性率为30％。检测结果表明:我们建立的生物素标记的HCMV DNA探针的点杂交法,具有高度的特异性、敏感性,比分离病毒法更迅速,可用于HCMV感染的临床标本的病毒核酸检测。
- 人巨细胞病毒
- , 生物素探针
- , 脐带血
- , 尿

Preparation and Application of Biotin-labelled Human Cytomegalovirus Nucleic Acid Probe

1.
Virus Researcli Institute
2.
Hubei Medical College

Abstract

A diagnostic assay has been developed to detect human cytomegalovirus (HCMV)DNA in clinical specimens with biotin-labelled DNA probe, cloned fragment of HCMV strain ADl69.The biotin-labelled probe can detect 100 pg of homologous DNA and does not hybridize with DNA from other herpesvirus or human embryonic lung cells(HEL) and Hep-2 cells. 30 umbilical cord blood and 10 urine specimens from pregnant women were examined by hybridization assay with biotin-labelled probe. Among 30 umbilical cord blood specimens,...
- Human Cytomegalovirus
- , Biotin-labelled
- , Umbilical cord blood
- , Urine

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Preparation and Application of Biotin-labelled Human Cytomegalovirus Nucleic Acid Probe

1. Virus Researcli Institute
2. Hubei Medical College

Abstract: A diagnostic assay has been developed to detect human cytomegalovirus (HCMV)DNA in clinical specimens with biotin-labelled DNA probe, cloned fragment of HCMV strain ADl69.The biotin-labelled probe can detect 100 pg of homologous DNA and does not hybridize with DNA from other herpesvirus or human embryonic lung cells(HEL) and Hep-2 cells. 30 umbilical cord blood and 10 urine specimens from pregnant women were examined by hybridization assay with biotin-labelled probe. Among 30 umbilical cord blood specimens,...