The Cloning and Characterization of the Genome of Human Papilloma Virus Type 16 in China

ZHANG Wei; JIN Shun-Qian; LIANG Xiao; SHANG Ming; SUN Jian-Heng; WANG Xi-Xia; TUN Ai-Ru

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February 1993

ZHANG Wei, JIN Shun-Qian, LIANG Xiao, SHANG Ming, SUN Jian-Heng, WANG Xi-Xia and TUN Ai-Ru. The Cloning and Characterization of the Genome of Human Papilloma Virus Type 16 in China[J]. Virologica Sinica, 1993, 8(1).

Citation: ZHANG Wei, JIN Shun-Qian, LIANG Xiao, SHANG Ming, SUN Jian-Heng, WANG Xi-Xia, TUN Ai-Ru. The Cloning and Characterization of the Genome of Human Papilloma Virus Type 16 in China .VIROLOGICA SINICA, 1993, 8(1) : 45.

我国人乳头瘤病毒(HPV16Z)基因克隆和鉴定

1.
中国医学科学院肿瘤所
2.
中国医学科学院肿瘤所北京

摘要

本文作者采用基因克隆手段,以pAt153为载体,从一份山西襄垣宫颈癌高发区宫颈癌患者的手术标本中,成功地克隆到2株与HPV16同源的基因片段。经PstI、KpnI、TaqvI、PvuII等16种限制性内切酶酶谱分析及其部分基因序列的鉴定,证明这是在国内首次克隆到一侏分子量约为8.0kb完整的HPV16型全序列DNA及一株分子量为5.4kb的HPV16基因片段。经实验证明:该基因片段的E6、E7及部分LI基因丢失,在750单核苷酸处发生变异,产生一新的BamHI酶切位点。该完整的HPV16基因组被命名为HPV16Z,HPV16基因片段被命名为HPV16F。用新分离到的HPV16Z作分子探针,检测襄垣337份宫颈癌及阴道活检标本的HPV16型同源序列的结果显示,慢性阴道炎阳性率为17.28％(14/81);宫颈炎为11.89％(17/143);宫颈癌前病变为46.81%(22/47);宫颈癌为72.73%(48/66)。证明山西宫颈癌高发区宫颈癌前病变及宫颈癌组织中主要为HPV16Z感染。
- 基因克隆
- , 人乳头瘤病毒16Z
- , 宫颈癌
- , 斑点杂交
- , 酶切图谱分析

The Cloning and Characterization of the Genome of Human Papilloma Virus Type 16 in China

1.
Institute of Oncology
2.
CAMS

Abstract

The cloning and characterization of a whole genome of human Papillomavirus type 16 (7.9kb) and a 5.4kb subgenomic fragment are presented here for the first time in China. Both of them were cloned in HB101 after insertion in plasmid pAt153, using it s unique BamH1 site. The full-length genome and subgenomic fragment are designated HPV16Z and HPV16F, respectively. After analysis of 16 endonucleases, none of new endonuclease site were found in HPV16Z comparing with standard strain. A new BamH1 site was observe...
- Human Papillomavirus
- , Cervical Cancer
- , Gene Clone
- , Dot-blot Hybridization

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Cloning and Characterization of the Genome of Human Papilloma Virus Type 16 in China

1. Institute of Oncology
2. CAMS

Abstract: The cloning and characterization of a whole genome of human Papillomavirus type 16 (7.9kb) and a 5.4kb subgenomic fragment are presented here for the first time in China. Both of them were cloned in HB101 after insertion in plasmid pAt153, using it s unique BamH1 site. The full-length genome and subgenomic fragment are designated HPV16Z and HPV16F, respectively. After analysis of 16 endonucleases, none of new endonuclease site were found in HPV16Z comparing with standard strain. A new BamH1 site was observe...