Experimental Infection of Hemorrhagic Fever with Renal Syndrome Virus in Mice and Viral Antigen Localization in Mouse Lymphocyte Subpopulations

TANG Yong-Ming; YANG Wei-Song; HONG Tao; ZHOU Jing-Yi

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June 1993

TANG Yong-Ming, YANG Wei-Song, HONG Tao and ZHOU Jing-Yi. Experimental Infection of Hemorrhagic Fever with Renal Syndrome Virus in Mice and Viral Antigen Localization in Mouse Lymphocyte Subpopulations[J]. Virologica Sinica, 1993, 8(3): 218-225.

Citation: TANG Yong-Ming, YANG Wei-Song, HONG Tao, ZHOU Jing-Yi. Experimental Infection of Hemorrhagic Fever with Renal Syndrome Virus in Mice and Viral Antigen Localization in Mouse Lymphocyte Subpopulations .VIROLOGICA SINICA, 1993, 8(3) : 218-225.

肾综合征出血热病毒对不同生理状态小鼠的实验感染及在小鼠淋巴细胞亚群中的定位

1.
第四军医大学唐都医院传染科
2.
中国预防医学科学院病毒研究所
3.
中国预防医学科学院病毒研究所西安

摘要

本文用双标记免疫细胞化学和双标记免疫电镜技术观察了环磷酰胺处理的BALB/c小鼠及乳鼠实验感染肾综合征出血热病毒后的发病和病毒定位情况,结果发现,免疫功能不成熟或有缺陷的小鼠感染后发病并有规律地死亡,而免疫功能正常鼠只呈隐性感染。在发病的和隐性感染的小鼠之间,病毒定位主要差别在于HFRSV是否感染免疫器官,即HFRSV抗原可见于发病鼠外周血,牌和胸腺的单个核细胞中,而在隐性感染小鼠的这些免疫器官中则为阴性,HFRS病毒颗粒及病毒抗原广泛见于发病鼠的T细胞亚群中,在Thy-1,L3T4和Lyt-2亚群中,双标记阳性细胞百分比分别为24.6+—15.3％,7.5％+—6.1％和17.7+—6.1％。对HFRSV在T细胞中分布的差异还作了动态比较。结果提示:细胞免疫可受HFRSV的直接影响,病毒对宿主免疫系统的感染是HFRSV感染发病的一个关键因素。
- 肾综合征出血热
- , 实验感染
- , 淋巴细胞亚群
- , 病毒定位
- , 双标记免疫细胞化学

Experimental Infection of Hemorrhagic Fever with Renal Syndrome Virus in Mice and Viral Antigen Localization in Mouse Lymphocyte Subpopulations

1.
Department of Infectious Diseases
2.
Tangdu Hospital
3.
The Fourth Military Meical University

Abstract

Double-immunoctytochemistry and double-immunoelectron microscopy were applied to investigate the pathogenesis and virus localization in cyclophosphamide-treated adult and suckling BALB/c mice af- ter the infection of hemorrhagic fever with renal syndrome virus. It was found that, after the infec tion, immunologically immature or deficient mice developed disease and died regularly, while normal adult mice showed only a subelinical infection. The major difference of virus localization between the diseased and...
- Hemorrhagic fever with renal syndrome
- , Experimental infection
- , Lymphocyte subpopulation
- , Virus localization

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Experimental Infection of Hemorrhagic Fever with Renal Syndrome Virus in Mice and Viral Antigen Localization in Mouse Lymphocyte Subpopulations

1. Department of Infectious Diseases
2. Tangdu Hospital
3. The Fourth Military Meical University

Abstract: Double-immunoctytochemistry and double-immunoelectron microscopy were applied to investigate the pathogenesis and virus localization in cyclophosphamide-treated adult and suckling BALB/c mice af- ter the infection of hemorrhagic fever with renal syndrome virus. It was found that, after the infec tion, immunologically immature or deficient mice developed disease and died regularly, while normal adult mice showed only a subelinical infection. The major difference of virus localization between the diseased and...