Western Blot Analysis of Viral Proteins Of Adult Diarrhea Rotavirus

FANG Zhao-Yin; WEN Le-Yang; R.Glass

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June 1993

FANG Zhao-Yin, WEN Le-Yang and R.Glass. Western Blot Analysis of Viral Proteins Of Adult Diarrhea Rotavirus[J]. Virologica Sinica, 1993, 8(3): 235-242.

Citation: FANG Zhao-Yin, WEN Le-Yang, R.Glass. Western Blot Analysis of Viral Proteins Of Adult Diarrhea Rotavirus .VIROLOGICA SINICA, 1993, 8(3) : 235-242.

成人腹泻轮状病毒蛋白的免疫印迹分析

1.
中国预防医学科学院病毒学研究所
2.
美国CDC 北京
3.
100052
4.
Atlanta
5.
GA 30333

摘要

我们从腹泻病人便样中纯化了病毒,其结构蛋白组分按分子量大小分别为VP1(136K),VP2(113K),VP3(92K),VP4(84K),VP5(64K),VP6(47K),VP7(41K)。所有这些蛋白皆具有抗原性。WP6是B组轮状病毒的共同抗原。B组轮状病毒的每一结构蛋白与A组轮状病毒都无交叉免疫反应。另外注意到二例不同病人对VP6和VP7刺激产生的抗体水平不同。
- 轮状病毒
- , 成人腹泻
- , 免疫印迹
- , 病毒蛋白
- , 免疫反应

Western Blot Analysis of Viral Proteins Of Adult Diarrhea Rotavirus

1.
1. Institute of Virology
2.
Chinese Academy of Preventive Medicine
3.
Beijing
4.
100052 2. Centers for Disease Control
5.
Allanta

Abstract

Adult diarrhea rotavirus (ADRV) is known as only one of group B rotavirus that causes human diarrhea. The viral proteins were determined by SDS-PAGE of purified virions from infected feces as follows: VP1 (136K), VP2 (113K), VP3 (92K), VP4 (84K), VP5 (64K), VP6 (47K), VP7(41K). Western blot analysis was used to examine their immunological properties. The results showed that all structural polypeptides are antigenic, VP6 is most strongly one and contains a common group B anti- gen. The rtatio of the antibod...
- Rotaviurs Adult diarrhea
- , Western blot
- , Viral protein
- , Immune responsibility

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Western Blot Analysis of Viral Proteins Of Adult Diarrhea Rotavirus

1. 1. Institute of Virology
2. Chinese Academy of Preventive Medicine
3. Beijing
4. 100052 2. Centers for Disease Control
5. Allanta

Abstract: Adult diarrhea rotavirus (ADRV) is known as only one of group B rotavirus that causes human diarrhea. The viral proteins were determined by SDS-PAGE of purified virions from infected feces as follows: VP1 (136K), VP2 (113K), VP3 (92K), VP4 (84K), VP5 (64K), VP6 (47K), VP7(41K). Western blot analysis was used to examine their immunological properties. The results showed that all structural polypeptides are antigenic, VP6 is most strongly one and contains a common group B anti- gen. The rtatio of the antibod...